Tingyu Ye1, Wei Ding2, Nanxiong Wang2, Hang Huang3, Yue Pan3, Anyang Wei4. 1. Department of Urology, Nanfang Hospital of Southern Medical University, Guangzhou, China; Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. 2. Department of Urology, Nanfang Hospital of Southern Medical University, Guangzhou, China. 3. Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. 4. Department of Urology, Nanfang Hospital of Southern Medical University, Guangzhou, China. Electronic address: profwei@126.com.
Abstract
BACKGROUND: More and more reports have demonstrated that long noncoding RNAs (lncRNAs) play an important role in the development of a variety of carcinomas, including bladder cancer. However, only a small fraction of them have been characterized. Linc00346 have been found to be upregulated in bladder cancer tissues compared to normal tissues in a microarray-based lncRNA profiling study. In this study, we would like to explore the expression pattern and functional role of linc00346 in bladder cancer. METHODS: We determined the expression of linc00346 in a cohort of bladder cancer tissues with matched normal tissues as well as human bladder cancer cell lines. We investigated the biological function of linc00346 with CCK-8 assay, colony formation assay, flow cytometry analysis, transwell assay and tumor xenografts mice model. RESULTS: We found that linc00346 was upregulated in bladder cancer tissues compared to normal tissues. Knockdown of linc00346 inhibited bladder cancer cell proliferation and migration, induced cell cycle arrest and cell apoptosis. CONCLUSION: Our study demonstrates that linc00346 could be a potential oncogene and a therapeutic target in bladder cancer.
BACKGROUND: More and more reports have demonstrated that long noncoding RNAs (lncRNAs) play an important role in the development of a variety of carcinomas, including bladder cancer. However, only a small fraction of them have been characterized. Linc00346 have been found to be upregulated in bladder cancer tissues compared to normal tissues in a microarray-based lncRNA profiling study. In this study, we would like to explore the expression pattern and functional role of linc00346 in bladder cancer. METHODS: We determined the expression of linc00346 in a cohort of bladder cancer tissues with matched normal tissues as well as humanbladder cancer cell lines. We investigated the biological function of linc00346 with CCK-8 assay, colony formation assay, flow cytometry analysis, transwell assay and tumor xenografts mice model. RESULTS: We found that linc00346 was upregulated in bladder cancer tissues compared to normal tissues. Knockdown of linc00346 inhibited bladder cancer cell proliferation and migration, induced cell cycle arrest and cell apoptosis. CONCLUSION: Our study demonstrates that linc00346 could be a potential oncogene and a therapeutic target in bladder cancer.
Authors: Rebecca Morgan; Willian Abraham da Silveira; Ryan Christopher Kelly; Ian Overton; Emma H Allott; Gary Hardiman Journal: Expert Rev Mol Diagn Date: 2021-11-25 Impact factor: 5.225