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Literature DB >> 287022

Purification of human platelet-derived growth factor.

Abstract

Human platelets contain a polypeptide growth factor that stimulates the proliferation of connective tissue cells. Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100 degrees C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtration in 1 M acetic acid, isoelectric focusing, and preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis. PDGF has an isoelectric point of 9.8 and a molecular weight ranging from 13,000 to 16,000 as judged by gel filtration in 1 M acetic acid or analytical sodium dodecyl sulfate gel electrophoresis under reducing conditions. The specific activity of the purified PDGF is 20 million times greater than that found in unfractionated human serum. Purified PDGF stimulates replicative DNA synthesis and cell proliferation in quiescent density-arrested cultures of BALB/c 3T3 cells at concentrations of 1 ng/ml (0.1 nM).

Entities: Chemical Gene Species

Mesh：

Substances：
Growth Substances

Year: 1979 PMID： 287022 PMCID： PMC383481 DOI： 10.1073/pnas.76.4.1809

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

1. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal.

Authors: S COHEN
Journal: J Biol Chem Date: 1962-05 Impact factor: 5.157

2. Partial purification and characterization of platelet factors stimulating the multiplication of normal human glial cells.

Authors: C H Heldin; A Wasteson; B Westermark
Journal: Exp Cell Res Date: 1977-10-15 Impact factor: 3.905

3. Induction of DNA synthesis in BALB/c 3T3 cells by serum components: reevaluation of the commitment process.

Authors: W J Pledger; C D Stiles; H N Antoniades; C D Scher
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

4. Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.

Authors: D Gospodarowicz
Journal: Nature Date: 1974-05-10 Impact factor: 49.962

5. Platelets as a source of fibroblast growth-promoting activity.

Authors: N Kohler; A Lipton
Journal: Exp Cell Res Date: 1974-08 Impact factor: 3.905

6. Stimulation of DNA synthesis and 2-deoxy-D-glucose transport in chick embryo cultures by excessive metal concentrations and by a carcinogenic hydrocarbon.

Authors: H Rubin; T Koide
Journal: J Cell Physiol Date: 1973-06 Impact factor: 6.384

7. Reversible denaturation of enzymes by sodium dodecyl sulfate.

Authors: K Weber; D J Kuter
Journal: J Biol Chem Date: 1971-07-25 Impact factor: 5.157

8. Growth factors derived from human serum, platelets, and pituitary: properties and immunologic cross-reactivity.

Authors: H N Antoniades; C D Scher
Journal: Natl Cancer Inst Monogr Date: 1978-05

9. Role of platelet factors in the growth of cells in culture.

Authors: R Ross; J Glomset; B Kariya; E Raines
Journal: Natl Cancer Inst Monogr Date: 1978-05

10. Radioimmunoassay of a human serum growth factor for Balb/c-3T3 cells: derivation from platelets.

Authors: H N Antoniades; C D Scher
Journal: Proc Natl Acad Sci U S A Date: 1977-05 Impact factor: 11.205

132 in total

1. Extraction and purification of TGFbeta and its effect on the induction of apoptosis of hepatocytes.

Authors: X H Si; L J Yang
Journal: World J Gastroenterol Date: 2001-08 Impact factor: 5.742

2. Platelet-derived growth factor stimulates synthesis of 1,2-diacylglycerol from monoacylglycerol in Balb/c 3T3 cells.

Authors: Y Hata; E Ogata; I Kojima
Journal: Biochem J Date: 1989-09-15 Impact factor: 3.857

3. Colostral growth factors. Possible role in bovine udder epithelial cell regeneration.

Authors: A Iivanainen; E Hölttä; A Ståhls; L C Andersson
Journal: Acta Vet Scand Date: 1992 Impact factor: 1.695

4. The 56 kd platelet-derived growth factor (PDGF)-related protein is phosphorylated and the most stable form in human glioma cells.

Authors: T Nakamura; I Takeshita; T Inamura; M Fukui
Journal: J Neurooncol Date: 1992-06 Impact factor: 4.130

5. Chitosan scaffold enhances growth factor release in wound healing in von Willebrand disease.

Authors: Mercy Halleluyah Periayah; Ahmad Sukari Halim; Arman Zaharil Mat Saad; Nik Soriani Yaacob; Abdul Rahim Hussein; Faraizah Abdul Karim; Ahmad Hazri Abdul Rashid; Zanariah Ujang
Journal: Int J Clin Exp Med Date: 2015-09-15

6. Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation.

Authors: P Pantazis; S Kharbanda; A S Goustin; T Galanopoulos; D Kufe
Journal: Proc Natl Acad Sci U S A Date: 1991-03-15 Impact factor: 11.205

7. Failure of epoprostenol (prostacyclin, PGI2) to inhibit platelet aggregation and to prevent restenosis after coronary angioplasty: results of a randomised placebo controlled trial.

Authors: A H Gershlick; D Spriggins; S W Davies; Y D Syndercombe Court; J Timmins; A D Timmis; M T Rothman; C Layton; R Balcon
Journal: Br Heart J Date: 1994-01

8. Human platelet-derived growth factor stimulates amino acid transport and protein synthesis by human diploid fibroblasts in plasma-free media.

Authors: A J Owen; R P Geyer; H N Antoniades
Journal: Proc Natl Acad Sci U S A Date: 1982-05 Impact factor: 11.205

9. Human platelet-derived growth factor (PDGF): purification of PDGF-I and PDGF-II and separation of their reduced subunits.

Authors: H N Antoniades
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

10. Structural and biological characteristics of connective tissue activating peptide (CTAP-III), a major human platelet-derived growth factor.

Authors: C W Castor; J W Miller; D A Walz
Journal: Proc Natl Acad Sci U S A Date: 1983-02 Impact factor: 11.205