Kunxue Deng1,2,3, Yaya Yang1,2,4, Yiquan Ke5, Chengyi Luo5, Man Liu6, Yuting Deng6, Quan Tian6, Yuyu Yuan6, Tun Yuan7, Tao Xu1,2. 1. a Department of Mechanical Engineering and Biomedical Engineering Program , University of Texas at El Paso , El Paso , TX , USA. 2. b Department of Mechanical Engineering , Bio-manufacturing Center, Tsinghua University , Beijing , China. 3. g Medprin Institute of Technology (MIT) , Guangzhou , China. 4. c Multidisciplinary Research Center, Shantou University , Shantou , China. 5. d Department of Neurosurgery , Zhujiang Hospital of South Medical University , Guangzhou , China. 6. e School of Bioscience & Bioengineering , South China University of Technology , Guangzhou , China. 7. f National Engineering Research Center for Biomaterials, Sichuan University , Chengdu , China.
Abstract
OBJECTIVE: Biomimetic design will significantly improve growth and regeneration of dural cells and tissue for better repairing effects and fewer complications in repairing the native dura. This study designed a novel composite, biomimetic substitute based on the characteristics of native dura extracellular matrix. METHODS AND RESULTS: This substitute is expected to rapidly induce cell adhesion, migration, and fast regeneration of neotissue. The material characteristics (contact angle, surface charge, and zeta potential were evaluated), in vitro biological characteristics (cell stretch, connections between cells, cell proliferation) and in vivo tissue regeneration capability of this substitute were evaluated, compared to those of collagen dura substitute, the mostly used dura substitute. The results showed that the surface properties of this composite substitute were more biomimetic to native extracellular matrix than collagen substitute did, together with better cytocompatibility, tissue ingrowth, and neoangiogenesis. This composite substitute further demonstrated in clinical case study its ideal repair effect with no CSF leakage or other adverse reactions. CONCLUSION: In conclusion, the new biomimetic composite substitute provides alternative substitute for dura repairing.
OBJECTIVE: Biomimetic design will significantly improve growth and regeneration of dural cells and tissue for better repairing effects and fewer complications in repairing the native dura. This study designed a novel composite, biomimetic substitute based on the characteristics of native dura extracellular matrix. METHODS AND RESULTS: This substitute is expected to rapidly induce cell adhesion, migration, and fast regeneration of neotissue. The material characteristics (contact angle, surface charge, and zeta potential were evaluated), in vitro biological characteristics (cell stretch, connections between cells, cell proliferation) and in vivo tissue regeneration capability of this substitute were evaluated, compared to those of collagen dura substitute, the mostly used dura substitute. The results showed that the surface properties of this composite substitute were more biomimetic to native extracellular matrix than collagen substitute did, together with better cytocompatibility, tissue ingrowth, and neoangiogenesis. This composite substitute further demonstrated in clinical case study its ideal repair effect with no CSF leakage or other adverse reactions. CONCLUSION: In conclusion, the new biomimetic composite substitute provides alternative substitute for dura repairing.