Cheng-Kun Yang1, Ting-Dong Yu1, Chuang-Ye Han1, Wei Qin1, Xi-Wen Liao1, Long Yu2, Xiao-Guang Liu3, Guang-Zhi Zhu1, Hao Su1, Si-Cong Lu1, Zhi-Wei Chen1, Zhen Liu1, Ke-Tuan Huang1, Zheng-Tao Liu1, Yu Liang1, Jian-Lu Huang4, Zeng-Nan Mo5, Xue Qin6, Lequn Li7, Kai-Yin Xiao1, Min-Hao Peng1, Cheryl Ann Winkle8, Stephen J O'Brien8, Tao Peng1. 1. Department of Hepatobiliary Surgery, Nanning, China. 2. Department of Hepatobiliary and Pancreatic Surgery, The first Affiliated Hospital of Zhengzhou University, Zhengzhou, China. 3. Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. 4. Department of Hepatobiliary Surgery, Third Affiliated Hospital of Guangxi Medical University, Nanning, China. 5. Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, China. 6. Department of Clinical Labaoratory, The first Affiliated Hospital of Guangxi Medical University, Nanning, China. 7. Department of Hepatobiliary Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China. 8. Laboratory of Genomic Diversity, National Cancer Institute, Frederick, Maryland, USA.
Abstract
BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is a common malignant tumor with a high rate of recurrence. Immunohistochemical analysis of the marker of proliferation Ki-67 (MKI67) is used to assess proliferation activity of HCC The regulation of MKI67 expression remains unclear in HCC This study aims to explore the association between MKI67 expression and gene variants. METHODS: A total of 195 hepatitis B virus (HBV)-related HCC patients were genotyped using Illumina HumanExome BeadChip-12-1_A (242,901 markers). An independent cohort (97 subjects) validated the association of polymorphism determinants and candidate genes with MKI67 expression. The relationships between MKI67 with p53 and variants of candidate genes in the clinical outcomes of HCC patients were analyzed. RESULTS: We found that MKI67 combined with p53 was associated with a 3-year recurrence-free survival and five variants near TTN and CCDC8 were associated with MKI67 expression. TTN harboring rs2288563-TT and rs2562832-AA+CA indicated a favorable outcome for HCC patients. CONCLUSION: Variants near TTN and CCDC8 were associated with MKI67 expression, and rs2288563 and rs2562832 in TTN are potential biomarkers for the prediction of clinical outcomes in HBV-related HCC patients.
BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is a common malignant tumor with a high rate of recurrence. Immunohistochemical analysis of the marker of proliferation Ki-67 (MKI67) is used to assess proliferation activity of HCC The regulation of MKI67 expression remains unclear in HCC This study aims to explore the association between MKI67 expression and gene variants. METHODS: A total of 195 hepatitis B virus (HBV)-related HCC patients were genotyped using Illumina HumanExome BeadChip-12-1_A (242,901 markers). An independent cohort (97 subjects) validated the association of polymorphism determinants and candidate genes with MKI67 expression. The relationships between MKI67 with p53 and variants of candidate genes in the clinical outcomes of HCC patients were analyzed. RESULTS: We found that MKI67 combined with p53 was associated with a 3-year recurrence-free survival and five variants near TTN and CCDC8 were associated with MKI67 expression. TTN harboring rs2288563-TT and rs2562832-AA+CA indicated a favorable outcome for HCC patients. CONCLUSION: Variants near TTN and CCDC8 were associated with MKI67 expression, and rs2288563 and rs2562832 in TTN are potential biomarkers for the prediction of clinical outcomes in HBV-related HCC patients.