Correction: Adipocyte arrestin domain-containing 3 protein (Arrdc3) regulates uncoupling protein 1 (Ucp1) expression in white adipose independently of canonical changes in β-adrenergic receptor signaling.

[This corrects the article DOI: 10.1371/journal.pone.0173823.].

Fig 1A is incorrect. Please see the entire correct Fig 1 here. Fig 2B is also incorrect. The western blot labeled “BAT” should read “VAT.” Please see the entire correct Fig 2 here.

Fig 1

Characterization of adipocyte-specific Arrdc3-null mice.

(A) To confirm adipocyte-specific deletion, Arrdc3 expression was measured in various tissues of Cre–(control) and Cre+ (Arrdc3-null) mice by quantitative PCR. Brown (BAT), parametrial (VAT) and subcutaneous adipose tissue (SAT) had significantly decreased Arrdc3 expression while there was no significant difference in liver or kidney (n = 3–4). (B) Adipocyte-specific Arrdc3-null mice and littermate controls were weighed for 16 weeks and no differences in body weight were found (n = 4–10). (C) Specific adipose depots of female mice were weighed and normalized to total body weight. Subcutaneous (SAT) and parametrial (VAT) adipose tissue from adipocyte-specific Arrdc3-null mice weighed significantly less than controls (n = 5). (D) Representative macroscopic (formaldehyde fixed tissue) and microscopic appearance of subcutaneous (SAT), parametrial (VAT) and brown (BAT) adipose tissue from adipocyte-specific Arrdc3-null and control mice. Paraffin tissue sections were stained with hematoxylin and eosin and images were taken at 40x.

Fig 2

Increased expression of Ucp1 in white adipose tissue of adipocyte-specific Arrdc3-null mice.

(A) Quantitative PCR analysis of gene expression in subcutaneous adipose tissue (n = 5–9). (B) Western analysis of Ucp1 protein expression in subcutaneous (SAT) and parametrial (VAT) adipose tissue. (C) 48 hours of CLAMS analysis of adipocyte-specific Arrdc3-null and control mice at 28°C ambient temperature (n = 5). *p≤ 0.05.