Ali Talha Khalil1,2,3, Muhammad Ovais1, Ikram Ullah1, Muhammad Ali1, Zabta Khan Shinwari1,4, Saleh Khamlich2,3, Malik Maaza2,3. 1. Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan. 2. UNESCO-UNISA Africa Chair in Nanoscience & Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, Gauteng, South Africa. 3. Nanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation Somerset West, Western Cape, South Africa. 4. Pakistan Academy of Sciences, Islamabad, Pakistan.
Abstract
AIM: To investigate the physical and biological properties of bioinspired zinc oxide (ZnO) nanoparticles via aqueous leaf extracts of Sageretia thea. EXPERIMENTAL: Nanoparticles of size approximately 12.4 nm were extensively characterized. In vitro antimicrobial, cytotoxic, biocompatible and enzyme inhibition assays were performed. RESULTS: Significant antimicrobial activities with and without UV illumination are reported. Bioinspired ZnO nanoparticles were found effective against fungal strains. MTT assay was performed to check the leishmanicidal activity against promastigotes (IC50: 6.2 μg/ml) and amastigotes (IC50: 10.87 μg/ml) of Leishmania tropica. Brine shrimp lethality was also indicated by bioinspired ZnO nanoparticles (IC50: 21.29 μg/ml). CONCLUSION: Hemocompatible nature of bioinspired nanoparticles was revealed. Furthermore, the antioxidant activities were performed. In addition, significant protein kinase while insignificant alpha amylase inhibition were recorded.
AIM: To investigate the physical and biological properties of bioinspired zinc oxide (ZnO) nanoparticles via aqueous leaf extracts of Sageretia thea. EXPERIMENTAL: Nanoparticles of size approximately 12.4 nm were extensively characterized. In vitro antimicrobial, cytotoxic, biocompatible and enzyme inhibition assays were performed. RESULTS: Significant antimicrobial activities with and without UV illumination are reported. Bioinspired ZnO nanoparticles were found effective against fungal strains. MTT assay was performed to check the leishmanicidal activity against promastigotes (IC50: 6.2 μg/ml) and amastigotes (IC50: 10.87 μg/ml) of Leishmania tropica. Brine shrimp lethality was also indicated by bioinspired ZnO nanoparticles (IC50: 21.29 μg/ml). CONCLUSION: Hemocompatible nature of bioinspired nanoparticles was revealed. Furthermore, the antioxidant activities were performed. In addition, significant protein kinase while insignificant alpha amylase inhibition were recorded.
Authors: Ahmad Faraz; Mohammad Faizan; Fareen Sami; Husna Siddiqui; John Pichtel; Shamsul Hayat Journal: IET Nanobiotechnol Date: 2019-06 Impact factor: 1.847