Yan-Zhao Chen1, Zhi-Ping Liu, Ke-Ying Zhou, Bo Li. 1. Department of Pediatrics, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518020, China. keyingzhou@163.com.
Abstract
OBJECTIVE: To investigate the value of serum miR-17-92 cluster in the diagnosis of retinoblastoma (RB). METHODS: Serum samples were collected from 20 children with RB and 20 healthy controls. Quantitative real-time PCR was used to measure the expression of miR-17-92 cluster. The expression of miR-17-92 cluster was compared between children with different stages of RB and the changes in the expression of miR-17-92 cluster after multimodality therapy were analyzed. The receiver operating characteristic (ROC) curve was used to investigate the value of serum miR-17-92 cluster in the diagnosis of RB. RESULTS: Compared with the healthy controls, the children with RB had significantly higher relative expression of miR-17-3P, miR-17-5P, miR-18a, and miR-20a in serum (P<0.05), and miR-18a showed the greatest increase. There were no significant differences in the relative expression of miR-19a, miR-19b-1, and miR-92a-1 between children with RB and healthy controls (P>0.05). There were no significant differences in the expression of miR-17-5P, miR-17-3P, miR-18a, and miR-20a between the children with early-to-moderate stage of RB and those with advanced stage of RB (P>0.05), but there were significant reductions after multimodality therapy (P<0.05). In the diagnosis of RB, the areas under the ROC curve (AUCs) for serum miR-17-3P, miR-17-5P, miR-18a, and miR-20a were 0.770, 0.755, 0.828, and 0.665 respectively, and miR-18a had the largest AUC, with a sensitivity of 90% and a specificity of 65%. CONCLUSIONS: miR-17-3P, miR-17-5P, miR-18a, and miR-20a are highly expressed in the serum of children with RB, and miR-18a may be used as a new marker for the diagnosis of RB.
OBJECTIVE: To investigate the value of serum miR-17-92 cluster in the diagnosis of retinoblastoma (RB). METHODS: Serum samples were collected from 20 children with RB and 20 healthy controls. Quantitative real-time PCR was used to measure the expression of miR-17-92 cluster. The expression of miR-17-92 cluster was compared between children with different stages of RB and the changes in the expression of miR-17-92 cluster after multimodality therapy were analyzed. The receiver operating characteristic (ROC) curve was used to investigate the value of serum miR-17-92 cluster in the diagnosis of RB. RESULTS: Compared with the healthy controls, the children with RB had significantly higher relative expression of miR-17-3P, miR-17-5P, miR-18a, and miR-20a in serum (P<0.05), and miR-18a showed the greatest increase. There were no significant differences in the relative expression of miR-19a, miR-19b-1, and miR-92a-1 between children with RB and healthy controls (P>0.05). There were no significant differences in the expression of miR-17-5P, miR-17-3P, miR-18a, and miR-20a between the children with early-to-moderate stage of RB and those with advanced stage of RB (P>0.05), but there were significant reductions after multimodality therapy (P<0.05). In the diagnosis of RB, the areas under the ROC curve (AUCs) for serum miR-17-3P, miR-17-5P, miR-18a, and miR-20a were 0.770, 0.755, 0.828, and 0.665 respectively, and miR-18a had the largest AUC, with a sensitivity of 90% and a specificity of 65%. CONCLUSIONS:miR-17-3P, miR-17-5P, miR-18a, and miR-20a are highly expressed in the serum of children with RB, and miR-18a may be used as a new marker for the diagnosis of RB.
Authors: Karina Conkrite; Maggie Sundby; Shizuo Mukai; J Michael Thomson; David Mu; Scott M Hammond; David MacPherson Journal: Genes Dev Date: 2011-08-04 Impact factor: 11.361
Authors: A MacCarthy; J M Birch; G J Draper; J L Hungerford; J E Kingston; M E Kroll; C A Stiller; T J Vincent; M F G Murphy Journal: Br J Ophthalmol Date: 2008-10-06 Impact factor: 4.638
Authors: David Nittner; Irina Lambertz; Frederic Clermont; Pieter Mestdagh; Corinna Köhler; Søren Jensby Nielsen; Aart Jochemsen; Frank Speleman; Jo Vandesompele; Michael A Dyer; Alexander Schramm; Johannes H Schulte; Jean-Christophe Marine Journal: Nat Cell Biol Date: 2012-08-05 Impact factor: 28.824