Lasse M Giil1,2, Christian A Vedeler3,4, Einar K Kristoffersen2,5, Jan Erik Nordrehaug2,6, Harald Heidecke7, Ralf Dechend8,9, Kai Schulze-Forster7, Dominik N Muller8,10, Victoria S von Goetze7, Otavio Cabral-Marques11, Gabriela Riemekasten11, Petra Vogelsang1,5, Staale Nygaard12, Anders Lund2, Dag Aarsland13,14. 1. Department of Internal Medicine, Haraldsplass Deaconess Hospital, Bergen, Norway. 2. Department of Clinical Science, University of Bergen, Bergen, Norway. 3. Department of Clinical Medicine, University of Bergen, Bergen, Norway. 4. Department of Neurology, Haukeland University Hospital, Bergen, Norway. 5. Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway. 6. Department of Cardiology, Stavanger University Hospital, Stavanger, Norway. 7. CellTrend GmbH, Luckenwalde, Berlin, Germany. 8. Experimental and Clinical Research Center, Charité Medical Faculty and the Max-Delbruck Center for Molecular Medicine, Berlin, Germany. 9. HELIOS-Klinikum Berlin, Berlin, Germany. 10. Max-Delbruck Center for Molecular Medicine, Berlin, Germany. 11. Department of Rheumatology, University Hospital Schleswig-Holstein, Lübeck, Germany. 12. Research Group for Biomedical Informatics, University of Oslo, Oslo, Norway. 13. Department of Old Age Psychiatry, Institute of Psychiatry, Psychology and Neuroscience, Kings College, UK. 14. Centre for Age-Related Diseases (SESAM), Stavanger University Hospital, Norway.
Abstract
BACKGROUND: Alzheimer's disease (AD) is associated with several antibodies as well as signaling molecules and receptors. These may be detrimental in the presence of a disrupted blood-brain barrier (BBB). OBJECTIVE: To investigate whether the levels of antibodies toward 33 signaling molecules involved in neurotransmitter, vascular, and immune functions were associated with AD and, within the AD group; cognitive function and mood. METHODS: Antibodies in sera from patients with mild AD [(n = 91) defined as a Mini-Mental State Examination ≥ 20 or a Clinical Dementia Rating Scale≤1] and healthy controls (n = 102) were measured with enzyme-linked immunosorbent assays. Levels in AD and controls were compared by Mann-Whitney test. In the AD group, associations between antibodies and psychometric test scores were analyzed by robust regression. The false discovery threshold was set to 0.05. RESULTS: Antibodies to serotonin receptors [5-HT2AR (effect size (r) = 0.21, p = 0.004), 5-HT2CR (r = 0.25, p = 0.0005) and 5-HT7R (r = 0.21, p = 0.003)], vascular endothelial growth factor receptor 1 [VEGFR1 (r = 0.29, p < 0.001)] and immune-receptors (Stabilin-1 (r = 0.23, p = 0.001) and C5aR1 (r = 0.21, p = 0.004) were higher in AD. Psychomotor speed was associated with D1R-abs (β 0.49, p < 0.001), depression with ETAR-abs (β 0.31, p < 0.001), and visuospatial function with 5-HT1AR-abs (β 0.27, p = 0.004) despite similar antibody levels compared to controls. CONCLUSIONS: Antibody levels to VEGFR1, serotonergic receptors, and receptors in the immune system were increased in AD. Antibodies at similar levels as in controls were associated cognitive dysfunction and depression in AD.
BACKGROUND:Alzheimer's disease (AD) is associated with several antibodies as well as signaling molecules and receptors. These may be detrimental in the presence of a disrupted blood-brain barrier (BBB). OBJECTIVE: To investigate whether the levels of antibodies toward 33 signaling molecules involved in neurotransmitter, vascular, and immune functions were associated with AD and, within the AD group; cognitive function and mood. METHODS: Antibodies in sera from patients with mild AD [(n = 91) defined as a Mini-Mental State Examination ≥ 20 or a Clinical Dementia Rating Scale≤1] and healthy controls (n = 102) were measured with enzyme-linked immunosorbent assays. Levels in AD and controls were compared by Mann-Whitney test. In the AD group, associations between antibodies and psychometric test scores were analyzed by robust regression. The false discovery threshold was set to 0.05. RESULTS: Antibodies to serotonin receptors [5-HT2AR (effect size (r) = 0.21, p = 0.004), 5-HT2CR (r = 0.25, p = 0.0005) and 5-HT7R (r = 0.21, p = 0.003)], vascular endothelial growth factor receptor 1 [VEGFR1 (r = 0.29, p < 0.001)] and immune-receptors (Stabilin-1 (r = 0.23, p = 0.001) and C5aR1 (r = 0.21, p = 0.004) were higher in AD. Psychomotor speed was associated with D1R-abs (β 0.49, p < 0.001), depression with ETAR-abs (β 0.31, p < 0.001), and visuospatial function with 5-HT1AR-abs (β 0.27, p = 0.004) despite similar antibody levels compared to controls. CONCLUSIONS: Antibody levels to VEGFR1, serotonergic receptors, and receptors in the immune system were increased in AD. Antibodies at similar levels as in controls were associated cognitive dysfunction and depression in AD.
Authors: Otavio Cabral-Marques; Alexandre Marques; Lasse Melvær Giil; Roberta De Vito; Judith Rademacher; Jeannine Günther; Tanja Lange; Jens Y Humrich; Sebastian Klapa; Susanne Schinke; Lena F Schimke; Gabriele Marschner; Silke Pitann; Sabine Adler; Ralf Dechend; Dominik N Müller; Ioana Braicu; Jalid Sehouli; Kai Schulze-Forster; Tobias Trippel; Carmen Scheibenbogen; Annetine Staff; Peter R Mertens; Madlen Löbel; Justin Mastroianni; Corinna Plattfaut; Frank Gieseler; Duska Dragun; Barbara Elizabeth Engelhardt; Maria J Fernandez-Cabezudo; Hans D Ochs; Basel K Al-Ramadi; Peter Lamprecht; Antje Mueller; Harald Heidecke; Gabriela Riemekasten Journal: Nat Commun Date: 2018-12-06 Impact factor: 14.919