| Literature DB >> 28695504 |
Rory K Morgan1, Michael S Cohen2.
Abstract
ADP-ribosylation, a posttranslational modification catalyzed by a family of enzymes known as poly(ADP-ribose) polymerases (PARPs, 17 in humans), regulates diverse cellular processes. To aid in understanding the functions of ADP-ribosylation in cells, we developed a clickable aminooxy probe, AO-alkyne, which detects ADP-ribosylation of acidic amino acids. AO-alkyne can be used to detect auto-ADP-ribosylation of PARP10 in cells following Cu-catalyzed click conjugation to an azide reporter. This method can be extended to other PARP family members that catalyze ADP-ribosylation on acidic amino acids, providing a convenient and direct readout of PARP activity in cells.Entities:
Keywords: ADP-ribosylation; Bioconjugation; Click chemistry; Oxime ligation; PARPs
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Year: 2017 PMID： 28695504 PMCID： PMC5920556 DOI： 10.1007/978-1-4939-6993-7_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745