L M Barin1, R C S Barcelos2, L T Vey3, F M Pillusky4, V M Palma5, K Z Kantorski6, M E Bürger7, R M Maciel4, C C Danesi8. 1. Post-Graduation Program in Dental Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: luisabarin@hotmail.com. 2. Post-Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil. 3. Post-Graduation Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil. 4. Post-Graduation Program in Dental Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil. 5. Department of Pathology, Federal University of Santa Maria, Santa Maria, RS, Brazil. 6. Post-Graduation Program in Dental Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil; Department of Stomatology, Federal University of Santa Maria, Santa Maria, RS, Brazil. 7. Post-Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Post-Graduation Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil. 8. Post-Graduation Program in Dental Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil; Department of Pathology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Abstract
BACKGROUND: To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model. METHODS: Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis. RESULTS: PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels. CONCLUSION: In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment.
BACKGROUND: To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model. METHODS:Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis. RESULTS: PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels. CONCLUSION: In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment.
Authors: Gerdal Roberto de Sousa; Leandro O Soares; Betânia M Soares; Rosana de Carvalho Cruz; Paulo Uliana Junior; Thiago Santiago; Luiz M Farias; Paula Prazeres Magalhães; Lívio B Silveira; Luciana Almeida Lopes; Marília Wellichan Mancini; Rudolf Huebner; Marcus V L Ferreira Journal: Lasers Med Sci Date: 2021-02-09 Impact factor: 3.161