Intissar Kammoun1,2, Intidhar Bkhairia3, Fatma Ben Abdallah4, Imen Jaballi1,5, Naourez Ktari3, Ons Boudawara6, Moncef Nasri3, Neji Gharsallah2, Ahmed Hakim5, Ibtissem Ben Amara1. 1. a Unit of Functional Genomics and Plant Physiology , Higher Institute of Biotechnology of Sfax , Sfax , Tunisia. 2. b Laboratory of Biotechnology Applied on Culture improvement of Plants (LR/01/ES-23), Sciences Faculty of Sfax , University of Sfax , Sfax , Tunisia. 3. c Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax , University of Sfax , Sfax , Tunisia. 4. d Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia. 5. e Laboratory of Pharmacology, Faculty of Medicine , University of Sfax , Sfax , Tunisia. 6. f Laboratory of Anatomopathology , CHU Habib Bourguiba, University of Sfax , Sfax , Tunisia.
Abstract
CONTEXT: Polysaccharides (PSs) from seaweeds have been reported to possess biological activity of potential medicinal values. OBJECTIVE: The current study was conducted to establish the protective effects of PS extracted from Ulva lactuca against oxidative stress induced by Thiacloprid (THC) in the rat reproductive system. MATERIALS AND METHODS: Rats were exposed either to THC, THC + PS (100 mg/kg), or THC + PS (200 mg/kg). RESULTS: Our study showed that THC induced severe disorders in the functional sperm parameters. A decrease in antioxidant activities and their genes expression were observed in the same group, compared to the controls. Our molecular data showing also a severe DNA breakdown in the testis of THC treated group. Moreover, THC treated group showed severe histopathological changes. CONCLUSIONS: Our results revealed that PS extracted from Ulva lactuca alleviated the THC induced reprotoxicity and reduced oxidative stress damages, DNA breakdown and histological injuries in the testis.
CONTEXT: Polysaccharides (PSs) from seaweeds have been reported to possess biological activity of potential medicinal values. OBJECTIVE: The current study was conducted to establish the protective effects of PS extracted from Ulva lactuca against oxidative stress induced by Thiacloprid (THC) in the rat reproductive system. MATERIALS AND METHODS:Rats were exposed either to THC, THC + PS (100 mg/kg), or THC + PS (200 mg/kg). RESULTS: Our study showed that THC induced severe disorders in the functional sperm parameters. A decrease in antioxidant activities and their genes expression were observed in the same group, compared to the controls. Our molecular data showing also a severe DNA breakdown in the testis of THC treated group. Moreover, THC treated group showed severe histopathological changes. CONCLUSIONS: Our results revealed that PS extracted from Ulva lactuca alleviated the THC induced reprotoxicity and reduced oxidative stress damages, DNA breakdown and histological injuries in the testis.