Xiaoyan Yang1, Jie Yin2, Qiong Xiang3, Hongyan Xie2, Jia Yu3, Runliang Gan4, Xiaoyong Lei5. 1. Institute of Biologic Research; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang Hunan 421001, China. 2. Institute of Biologic Research, University of South China, Hengyang Hunan 421001, China. 3. Institute of Pharmacy and Pharmacology, University of South China, Hengyang Hunan 421001, China. 4. Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study; Cancer Research Institute, University of South China, Hengyang Hunan 421001, China. 5. Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study; Institute of Pharmacy and Pharmacology, University of South China, Hengyang Hunan 421001, China.
Abstract
OBJECTIVE: To investigate effects of miR-503 on cisplatin sensitivity in BEL-7402 cells by targeting of bcl-2. Methods: MiR-503 and bcl-2 mRNA expression levels in hepatocellular carcinoma cells were measured by real-time quantitative (qRT)-PCR; Bcl-protein level was detected by Western blot; miR-503 mimics were transiently transfected to the BEL-7402 cells by liposome transfection; potential target genes of miR-503 were predicted by Bioinformatics software; miR-503 potential targets were validated by dual luciferase activity; and the cell viability was measured by MTT assay. Results: MiR-503 level was down-regulated and Bcl-2 protein expression level was up-regulated in BEL-7402 cells compared with HL-7702 cells. MiR-503 could interact with bcl-2 and inhibit its expression. Cell vitality with miR-503 transfection was significantly reduced compared to that in the negative control. Conclusion: MiR-503 may enhance the sensitivity of BEL-7402 cells to cisplatin and inhibit the cell proliferation by targeting bcl-2.
OBJECTIVE: To investigate effects of miR-503 on cisplatin sensitivity in BEL-7402 cells by targeting of bcl-2. Methods:MiR-503 and bcl-2 mRNA expression levels in hepatocellular carcinoma cells were measured by real-time quantitative (qRT)-PCR; Bcl-protein level was detected by Western blot; miR-503 mimics were transiently transfected to the BEL-7402 cells by liposome transfection; potential target genes of miR-503 were predicted by Bioinformatics software; miR-503 potential targets were validated by dual luciferase activity; and the cell viability was measured by MTT assay. Results:MiR-503 level was down-regulated and Bcl-2 protein expression level was up-regulated in BEL-7402 cells compared with HL-7702 cells. MiR-503 could interact with bcl-2 and inhibit its expression. Cell vitality with miR-503 transfection was significantly reduced compared to that in the negative control. Conclusion:MiR-503 may enhance the sensitivity of BEL-7402 cells to cisplatin and inhibit the cell proliferation by targeting bcl-2.