Lewan Parker1, Adam Trewin2, Itamar Levinger3, Christopher S Shaw4, Nigel K Stepto5. 1. Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia. Electronic address: lewan.parker@vu.edu.au. 2. Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia. 3. Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia; Australian Institute for Musculoskeletal Science (AIMSS), Australia. 4. Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia; Institute for Physical Activity and Nutrition, School of Exercise and Nutrition Sciences, Deakin University, Australia. 5. Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Australia; Australian Institute for Musculoskeletal Science (AIMSS), Australia; Monash Centre for Health Research and Implementation (MCHRI), School of Public Health and Preventative Medicine, Monash University and Monash Health, Australia.
Abstract
OBJECTIVES: Redox homeostasis and redox-sensitive protein signaling play a role in exercise-induced adaptation. The effects of sprint-interval exercise (SIE), high-intensity interval exercise (HIIE) and continuous moderate-intensity exercise (CMIE), on post-exercise plasma redox status are unclear. Furthermore, whether post-exercise plasma redox status reflects skeletal muscle redox-sensitive protein signaling is unknown. DESIGN: In a randomized crossover design, eight healthy adults performed a cycling session of HIIE (5×4min at 75% Wmax), SIE (4×30s Wingate's), and CMIE work-matched to HIIE (30min at 50% of Wmax). METHODS:Plasma hydrogen peroxide (H2O2), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD) activity, and catalase activity were measured immediately post, 1h, 2h and 3h post-exercise. Plasma redox status biomarkers were correlated with phosphorylation of skeletal muscle p38-MAPK, JNK, NF-κB, and IκBα protein content immediately and 3h post-exercise. RESULTS:Plasma catalase activity was greater with SIE (56.6±3.8Uml-1) compared to CMIE (42.7±3.2, p<0.01) and HIIE (49.0±5.5, p=0.07). Peak plasma H2O2 was significantly (p<0.05) greater after SIE (4.6±0.6nmol/ml) and HIIE (4.1±0.4) compared to CMIE (3.3±0.5). Post-exercise plasma TBARS and SOD activity significantly (p<0.05) decreased irrespective of exercise protocol. A significant positive correlation was detected between plasma catalase activity and skeletal muscle p38-MAPK phosphorylation 3h post-exercise (r=0.40, p=0.04). No other correlations were detected (all p>0.05). CONCLUSIONS: Low-volume SIE elicited greater post-exercise plasma catalase activity compared to HIIE and CMIE, and greater H2O2 compared to CMIE. Plasma redox status did not, however, adequately reflect skeletal muscle redox-sensitive protein signaling.
RCT Entities:
OBJECTIVES: Redox homeostasis and redox-sensitive protein signaling play a role in exercise-induced adaptation. The effects of sprint-interval exercise (SIE), high-intensity interval exercise (HIIE) and continuous moderate-intensity exercise (CMIE), on post-exercise plasma redox status are unclear. Furthermore, whether post-exercise plasma redox status reflects skeletal muscle redox-sensitive protein signaling is unknown. DESIGN: In a randomized crossover design, eight healthy adults performed a cycling session of HIIE (5×4min at 75% Wmax), SIE (4×30s Wingate's), and CMIE work-matched to HIIE (30min at 50% of Wmax). METHODS: Plasma hydrogen peroxide (H2O2), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD) activity, and catalase activity were measured immediately post, 1h, 2h and 3h post-exercise. Plasma redox status biomarkers were correlated with phosphorylation of skeletal muscle p38-MAPK, JNK, NF-κB, and IκBα protein content immediately and 3h post-exercise. RESULTS: Plasma catalase activity was greater with SIE (56.6±3.8Uml-1) compared to CMIE (42.7±3.2, p<0.01) and HIIE (49.0±5.5, p=0.07). Peak plasma H2O2 was significantly (p<0.05) greater after SIE (4.6±0.6nmol/ml) and HIIE (4.1±0.4) compared to CMIE (3.3±0.5). Post-exercise plasma TBARS and SOD activity significantly (p<0.05) decreased irrespective of exercise protocol. A significant positive correlation was detected between plasma catalase activity and skeletal muscle p38-MAPK phosphorylation 3h post-exercise (r=0.40, p=0.04). No other correlations were detected (all p>0.05). CONCLUSIONS: Low-volume SIE elicited greater post-exercise plasma catalase activity compared to HIIE and CMIE, and greater H2O2 compared to CMIE. Plasma redox status did not, however, adequately reflect skeletal muscle redox-sensitive protein signaling.
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