Jing Li1, Jin Liang Ping2, Bo Ma3, Ying Rong Chen1, Li Qin Li4. 1. Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Affiliated with Zhejiang University, Huzhou, 313000, China. 2. Department of Pathology, Huzhou Central Hospital Affiliated with Zhejiang University, Huzhou, 313000, China. 3. Department of Surgery, Zhejiang Hospital, Hangzhou, 313000, China. 4. Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Affiliated with Zhejiang University, Huzhou, 313000, China. Electronic address: Lililiqinhust@126.com.
Abstract
INTRODUCTION: The aim of this study was to investigate miR-126-3p expression in stroma and tumor cells of basal-like breast cancer tissues, in an effort to elucidate the potential effect of miR-126-3p on tumor microenvironment and progress of basal-like breast cancer. METHODS: Expression levels of miR-126-3p in 33 paired basal-like breast cancer tissues were assayed by real-time quantitative PCR. Tumor cells and normal epithelial cell were isolated from ten paired basal-like breast cancer tissues and matched adjacent tissues, separately, using laser capture microdissect(LCM)-based PCR method. Further validated in larger sets were assayed by tissue microarrays (TMA)-based ISH method. RESULTS: MiR-126-3p expression level had no significant differences between basal-like breast cancer subtypes and matched adjacent tissues. However, a decreasing trend of miR-126-3p expression can be found in tumor cells of basal-like subtype, compared with matched adjacent tissues, using LCM-based PCR. Using TMA method, miR-126-3p expression level was the lowest in stroma of basal-like breast cancers among four subtypes (χ2=10.55, P=0.01), and was increasing in stroma of breast cancers compared with fibroadenomas. Furthermore, strong miR-126-3p expression in stroma is significantly associated with HER-2 expression (χ2=4.70, P=0.03) and Ki-67 index. (χ2=4.84, P=0.03), which suggested a potential prognostic value of miR-126-3p in stroma of breast cancer. However, miR-126-3p expression in tumor cells derived from different subtypes hadn't significant clinical values in this study. CONCLUSIONS: the miR-126-3p expression level in breast cancer stroma was associated with different intrinsic subtypes and its correlation with hormone receptor and Ki-67 index shed light on the potential clinical prognostic value of miR-126-3p, in the field of specific breast cancer subtypes.
INTRODUCTION: The aim of this study was to investigate miR-126-3p expression in stroma and tumor cells of basal-like breast cancer tissues, in an effort to elucidate the potential effect of miR-126-3p on tumor microenvironment and progress of basal-like breast cancer. METHODS: Expression levels of miR-126-3p in 33 paired basal-like breast cancer tissues were assayed by real-time quantitative PCR. Tumor cells and normal epithelial cell were isolated from ten paired basal-like breast cancer tissues and matched adjacent tissues, separately, using laser capture microdissect(LCM)-based PCR method. Further validated in larger sets were assayed by tissue microarrays (TMA)-based ISH method. RESULTS:MiR-126-3p expression level had no significant differences between basal-like breast cancer subtypes and matched adjacent tissues. However, a decreasing trend of miR-126-3p expression can be found in tumor cells of basal-like subtype, compared with matched adjacent tissues, using LCM-based PCR. Using TMA method, miR-126-3p expression level was the lowest in stroma of basal-like breast cancers among four subtypes (χ2=10.55, P=0.01), and was increasing in stroma of breast cancers compared with fibroadenomas. Furthermore, strong miR-126-3p expression in stroma is significantly associated with HER-2 expression (χ2=4.70, P=0.03) and Ki-67 index. (χ2=4.84, P=0.03), which suggested a potential prognostic value of miR-126-3p in stroma of breast cancer. However, miR-126-3p expression in tumor cells derived from different subtypes hadn't significant clinical values in this study. CONCLUSIONS: the miR-126-3p expression level in breast cancer stroma was associated with different intrinsic subtypes and its correlation with hormone receptor and Ki-67 index shed light on the potential clinical prognostic value of miR-126-3p, in the field of specific breast cancer subtypes.