Specificity of prostaglandin D2 binding to synaptic membrane fraction of rat brain.

The structural requirement of the prostaglandin D2 molecule for binding to the synaptic membrane fraction of rat brain was extensively studied by using various prostaglandin D derivatives. Most strict specificity was found in the structures of the cyclo-pentane ring and the double bond in 13,14-position. The addition and deprivation of the double bond in alpha- and omega-chain, except on 13,14-position, moderately affected the binding. The modification in the carboxyl terminus and omega-chain terminus did not seriously influence the binding. BW 245C and 9-beta-prostaglandin D2, potent agonists for the prostaglandin D2 receptor in the platelet membrane, were almost ineffective. [3H]prostaglandin D2 binding was not affected by the addition of various neuroactive substances to the binding assay mixture. Further, prostaglandin D2 did not affect the known neurotransmitter receptor bindings in the rat brain.