Kazuya Takamochi1, Fumiyuki Takahashi2, Yoshiyuki Suehara3, Eiichi Sato4, Shinji Kohsaka5, Takuo Hayashi6, Shigehisa Kitano7, Toshihide Uneno8, Shinya Kojima8, Kengo Takeuchi9, Hiroyuki Mano10, Kenji Suzuki11. 1. Department of General Thoracic Surgery, Juntendo University School of Medicine, Japan. Electronic address: ktakamo@juntendo.ac.jp. 2. Department of Respiratory Medicine, Juntendo University School of Medicine, Japan. 3. Department of Orthopedic Surgery, Juntendo University School of Medicine, Japan. 4. Department of Pathology, Institute of Medical Science (Medical Research Center), Tokyo Medical University, Japan. 5. Department of Medical Genomics, Graduate School of Medicine, The University of Tokyo, Japan. 6. Department of Human Pathology, Juntendo University School of Medicine, Japan. 7. Department of Experimental Therapeutics, National Cancer Center Hospital, Japan. 8. Departments of Cellular Signaling, Graduate School of Medicine, The University of Tokyo, Japan. 9. Pathology Project for Molecular Targets, The Cancer Institute, Japanese Foundation for Cancer Research, Japan. 10. Departments of Cellular Signaling, Graduate School of Medicine, The University of Tokyo, Japan; National Cancer Center Research Institute, Tokyo, Japan. 11. Department of General Thoracic Surgery, Juntendo University School of Medicine, Japan.
Abstract
OBJECTIVES: DNA mismatch repair (MMR) deficiency has recently received increasing attention as a significant biomarker to predict the treatment effect of immune checkpoint inhibitors for various malignant neoplasms. To evaluate MMR status, we analyzed the microsatellite instability (MSI) of lung adenocarcinomas. MATERIALS AND METHODS: Frozen tissues of lung adenocarcinoma and corresponding normal lung were obtained from 341 patients, including 141 with tumors harboring driver gene alterations (50 EGFR gene mutations, 50 KRAS gene mutations, 21 ALK fusions, 10 ROS1 fusions, and 10 RET fusions) and 200 with pan-negative tumors (100 never- or light-smokers and 100 heavy-smokers), who were surgically treated between 2007 and 2015. Genomic DNA extracted from tumors and corresponding normal lung tissues were used for MSI analysis using the Promega panel (5 mononucleotide markers: BAT-25, BAT-26, NR-21, NR-24, and MONO-27; and 2 pentanucleotide markers: Penta C and Penta D). RESULTS: MSI was identified in only 1 pan-negative tumor from a 64-year-old male heavy smoker. MSI was found in 4 mononucleotide markers. Although no clinical background of Lynch syndrome was evident, somatic MLH1 gene mutation was identified. MLH1 was expressed in tumor-infiltrating lymphocytes and was not expressed in cancer cells. PD-L1 was not expressed in cancer cells, and PD-1 was not expressed in tumor-infiltrating lymphocytes. CONCLUSION: MSI is a rare event in lung adenocarcinoma regardless of smoking status and mutation status of driver oncogenes. Accordingly, MMR deficiency status cannot be used as a biomarker for immune checkpoint inhibitor treatment for lung adenocarcinoma.
OBJECTIVES: DNA mismatch repair (MMR) deficiency has recently received increasing attention as a significant biomarker to predict the treatment effect of immune checkpoint inhibitors for various malignant neoplasms. To evaluate MMR status, we analyzed the microsatellite instability (MSI) of lung adenocarcinomas. MATERIALS AND METHODS: Frozen tissues of lung adenocarcinoma and corresponding normal lung were obtained from 341 patients, including 141 with tumors harboring driver gene alterations (50 EGFR gene mutations, 50 KRAS gene mutations, 21 ALK fusions, 10 ROS1 fusions, and 10 RET fusions) and 200 with pan-negative tumors (100 never- or light-smokers and 100 heavy-smokers), who were surgically treated between 2007 and 2015. Genomic DNA extracted from tumors and corresponding normal lung tissues were used for MSI analysis using the Promega panel (5 mononucleotide markers: BAT-25, BAT-26, NR-21, NR-24, and MONO-27; and 2 pentanucleotide markers: Penta C and Penta D). RESULTS: MSI was identified in only 1 pan-negative tumor from a 64-year-old male heavy smoker. MSI was found in 4 mononucleotide markers. Although no clinical background of Lynch syndrome was evident, somatic MLH1 gene mutation was identified. MLH1 was expressed in tumor-infiltrating lymphocytes and was not expressed in cancer cells. PD-L1 was not expressed in cancer cells, and PD-1 was not expressed in tumor-infiltrating lymphocytes. CONCLUSION: MSI is a rare event in lung adenocarcinoma regardless of smoking status and mutation status of driver oncogenes. Accordingly, MMR deficiency status cannot be used as a biomarker for immune checkpoint inhibitor treatment for lung adenocarcinoma.