Wang Zhongchao1, Fan Liyuan2, Tan Dan1, Zhou Cong1, Luo Shijun2. 1. Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, China;Dept. of Oral Medicine, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China. 2. Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, China;Dept. of Prosthodontics, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China.
Abstract
OBJECTIVE: To explore the therapeutic effect of enhancer of Zeste homolog 2 (EZH2) inhibitor GSK343 on periodontitis by regulating microphage differentiation. METHODS: Macrophage RAW264.7 cells were divided into the blank (A group), control (B group), lipopolysaccharide (LPS) stimulation (C group), and LPS+GSK343 (D group) groups. Phenotype transformations was determined through Western blot analysis and enzyme-linked immunosorbent assay by detecting the differentiation of phenotypic biological markers, including tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), interleukin-10 (IL-10), and Arginase-1 (Arg-1). Metergasis was identified by performing a phagocytosis test on Escherichia coli (E. coli). RESULTS: Macrophage RAW264.7 cells produced classical phenotypic biomarkers (M1) TNF-α and iNOS under LPS stimulation. The expression levels of IL-10 and Arg-1 increased after adding GSK343 into the culture medium. GSK343 also induced the conversion of M1 macrophages into M2 macrophages. Macrophage RAW264.7 cells exerted a phagocytic effect on E. coli, and this effect was enhanced after adding LPS into the culture medium. GSK343 regulated the macrophage RAW264.7 phagocytosis of E. coli. CONCLUSIONS: GSK343 possibly participates in the regulation of macrophage differentiation and, consequently, in the latent treatment of periodontitis.
OBJECTIVE: To explore the therapeutic effect of enhancer of Zeste homolog 2 (EZH2) inhibitor GSK343 on periodontitis by regulating microphage differentiation. METHODS: Macrophage RAW264.7 cells were divided into the blank (A group), control (B group), lipopolysaccharide (LPS) stimulation (C group), and LPS+GSK343 (D group) groups. Phenotype transformations was determined through Western blot analysis and enzyme-linked immunosorbent assay by detecting the differentiation of phenotypic biological markers, including tumornecrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), interleukin-10 (IL-10), and Arginase-1 (Arg-1). Metergasis was identified by performing a phagocytosis test on Escherichia coli (E. coli). RESULTS: Macrophage RAW264.7 cells produced classical phenotypic biomarkers (M1) TNF-α and iNOS under LPS stimulation. The expression levels of IL-10 and Arg-1 increased after adding GSK343 into the culture medium. GSK343 also induced the conversion of M1 macrophages into M2 macrophages. Macrophage RAW264.7 cells exerted a phagocytic effect on E. coli, and this effect was enhanced after adding LPS into the culture medium. GSK343 regulated the macrophage RAW264.7 phagocytosis of E. coli. CONCLUSIONS:GSK343 possibly participates in the regulation of macrophage differentiation and, consequently, in the latent treatment of periodontitis.
Authors: Irina Alimova; Sujatha Venkataraman; Peter Harris; Victor E Marquez; Paul A Northcott; Adrian Dubuc; Michael D Taylor; Nicholas K Foreman; Rajeev Vibhakar Journal: Int J Cancer Date: 2012-07-30 Impact factor: 7.396
Authors: P Stenvinkel; M Karimi; S Johansson; J Axelsson; M Suliman; B Lindholm; O Heimbürger; P Barany; A Alvestrand; L Nordfors; A R Qureshi; T J Ekström; M Schalling Journal: J Intern Med Date: 2007-05 Impact factor: 8.989
Authors: E Susol; A L Rands; A Herrick; N McHugh; J H Barrett; W E Ollier; J Worthington Journal: Rheumatology (Oxford) Date: 2000-12 Impact factor: 7.580
Authors: Clinton D Kemp; Mahadev Rao; Sichuan Xi; Suzanne Inchauste; Haresh Mani; Patricia Fetsch; Armando Filie; Mary Zhang; Julie A Hong; Robert L Walker; Yuelin J Zhu; R Taylor Ripley; Aarti Mathur; Fang Liu; Maocheng Yang; Paul A Meltzer; Victor E Marquez; Assunta De Rienzo; Raphael Bueno; David S Schrump Journal: Clin Cancer Res Date: 2011-10-25 Impact factor: 12.531