| Literature DB >> 28674906 |
Ana Sofia Carvalho1, Rune Matthiesen2.
Abstract
Bronchoalveolar lavage (BAL) fluid, obtained by a relatively noninvasive procedure, is used as a practice for diagnosis of various lung diseases as source of cells for cytology analysis. The acellular component of BAL potentially can complement and be a key for the establishment of diagnostic or as a prognostic indicator. This chapter discusses the aspects of standardization of BAL sample preparation and processing and its implications on the BAL fluid proteome quantitative analysis by high-throughput mass spectrometry. The detailed conditions for quantitative analysis of BAL proteome in the context of biomarker discovery are introduced.Entities:
Keywords: Biomarkers; Bronchoalveolar lavage fluid; Diagnosis; Lung diseases; Mass spectrometry; Proteome
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Year: 2017 PMID： 28674906 DOI： 10.1007/978-1-4939-7057-5_34
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745