Literature DB >> 28670603

In vitro Detection of Neutrophil Traps and Post-attack Cell Wall Changes in Candida Hyphae.

Alex Hopke1, Robert T Wheeler1,2.   

Abstract

In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell wall, making them promising tools for exploring host-pathogen interactions during fungal infection.

Entities:  

Keywords:  C. albicans; Cell wall; Fungi; Host-pathogen interactions; NETs; sDectin-1-Fc

Year:  2017        PMID： 28670603      PMCID： PMC5493211          DOI： 10.21769/BioProtoc.2213

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  12 in total

Review 1.  Neutrophil function: from mechanisms to disease.

Authors:  Borko Amulic; Christel Cazalet; Garret L Hayes; Kathleen D Metzler; Arturo Zychlinsky
Journal:  Annu Rev Immunol       Date:  2012-01-03       Impact factor: 28.527

2.  Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA.

Authors:  Sandra Bruns; Olaf Kniemeyer; Mike Hasenberg; Vishukumar Aimanianda; Sandor Nietzsche; Andreas Thywissen; Andreas Jeron; Jean-Paul Latgé; Axel A Brakhage; Matthias Gunzer
Journal:  PLoS Pathog       Date:  2010-04-29       Impact factor: 6.823

3.  NADPH oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis.

Authors:  Marc Röhm; Melissa J Grimm; Anthony C D'Auria; Nikolaos G Almyroudis; Brahm H Segal; Constantin F Urban
Journal:  Infect Immun       Date:  2014-02-18       Impact factor: 3.441

4.  Neutrophil extracellular traps capture and kill Candida albicans yeast and hyphal forms.

Authors:  Constantin F Urban; Ulrike Reichard; Volker Brinkmann; Arturo Zychlinsky
Journal:  Cell Microbiol       Date:  2006-04       Impact factor: 3.715

5.  Isolation, purification and labeling of mouse bone marrow neutrophils for functional studies and adoptive transfer experiments.

Authors:  Muthulekha Swamydas; Michail S Lionakis
Journal:  J Vis Exp       Date:  2013-07-10       Impact factor: 1.355

Review 6.  Hidden killers: human fungal infections.

Authors:  Gordon D Brown; David W Denning; Neil A R Gow; Stuart M Levitz; Mihai G Netea; Theodore C White
Journal:  Sci Transl Med       Date:  2012-12-19       Impact factor: 17.956

Review 7.  Molecular mechanisms regulating NETosis in infection and disease.

Authors:  Nora Branzk; Venizelos Papayannopoulos
Journal:  Semin Immunopathol       Date:  2013-06-04       Impact factor: 9.623

8.  Candida and host determinants of susceptibility to invasive candidiasis.

Authors:  Michail S Lionakis; Mihai G Netea
Journal:  PLoS Pathog       Date:  2013-01-03       Impact factor: 6.823

9.  Dynamic, morphotype-specific Candida albicans beta-glucan exposure during infection and drug treatment.

Authors:  Robert T Wheeler; Diana Kombe; Sudeep D Agarwala; Gerald R Fink
Journal:  PLoS Pathog       Date:  2008-12-05       Impact factor: 6.823

10.  Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition.

Authors:  Alex Hopke; Nadine Nicke; Erica E Hidu; Genny Degani; Laura Popolo; Robert T Wheeler
Journal:  PLoS Pathog       Date:  2016-05-25       Impact factor: 6.823
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  2 in total

1.  Control of Mucosal Candidiasis in the Zebrafish Swim Bladder Depends on Neutrophils That Block Filament Invasion and Drive Extracellular-Trap Production.

Authors:  Remi L Gratacap; Allison K Scherer; Brittany G Seman; Robert T Wheeler
Journal:  Infect Immun       Date:  2017-08-18       Impact factor: 3.441

2.  Spleen Tyrosine Kinase Is a Critical Regulator of Neutrophil Responses to Candida Species.

Authors:  Paige E Negoro; Shuying Xu; Zeina Dagher; Alex Hopke; Jennifer L Reedy; Michael B Feldman; Nida S Khan; Adam L Viens; Natalie J Alexander; Natalie J Atallah; Allison K Scherer; Richard A Dutko; Jane Jeffery; John F Kernien; J Scott Fites; Jeniel E Nett; Bruce S Klein; Jatin M Vyas; Daniel Irimia; David B Sykes; Michael K Mansour
Journal:  mBio       Date:  2020-05-12       Impact factor: 7.786
  2 in total

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