Jinling Fu1, Jingxue Jiao1, Kechao Weng2, Dan Yu3, Ruijun Li4. 1. Department of Ophthalmology, The Second Hospital of Jilin UniversityChangchun 130041, Jilin, China. 2. Eye Center of The Second Affiliated Hospital School of Medicine, Institute of Translational Medicine, Zhejiang UniversityHangzhou 310058, Zhejiang, China. 3. Department of Otolaryngology Head and Neck Surgery, The Second Hospital of Jilin UniversityChangchun 130041, Jilin, China. 4. Department of Hand Surgery, The First Hospital of Jilin UniversityChangchun 130021, Jilin, China.
Abstract
PURPOSE: Methanol exposure have been shown to produce retinal abnormalities and visual dysfunctions in rodents and other mammals developing in utero. In this study, we characterized how methanol affects the retinal development in an ex utero embryonic system, the zebrafish. METHODS: Zebrafish embryos were raised for 24 hours in fish water supplemented with various concentrations of methanol at 6 hours after fertilisation. The effects of methanol on retinal morphology were assessed by histologic and immunohistochemical analyses. RESULTS: Zebrafish embryos exposed to moderate (3%) and high (4%) levels of methanol during early embryonic development had a small eye phenotype. Embryos exposed to high (4%) level of methanol had morphological abnormalities of the retinal pigment epithelia and the photoreceptors. Methanol exposure also caused inhibition of cell differentiation and proliferation in the retina at the early developmental stage. CONCLUSIONS: Low concentrations of methanol affect photoreceptor function but do not disturb retinal morphology. Higher levels of methanol exposure cause retinal patterning defects and a small eye phenotype.
PURPOSE:Methanol exposure have been shown to produce retinal abnormalities and visual dysfunctions in rodents and other mammals developing in utero. In this study, we characterized how methanol affects the retinal development in an ex utero embryonic system, the zebrafish. METHODS:Zebrafish embryos were raised for 24 hours in fish water supplemented with various concentrations of methanol at 6 hours after fertilisation. The effects of methanol on retinal morphology were assessed by histologic and immunohistochemical analyses. RESULTS:Zebrafish embryos exposed to moderate (3%) and high (4%) levels of methanol during early embryonic development had a small eye phenotype. Embryos exposed to high (4%) level of methanol had morphological abnormalities of the retinal pigment epithelia and the photoreceptors. Methanol exposure also caused inhibition of cell differentiation and proliferation in the retina at the early developmental stage. CONCLUSIONS: Low concentrations of methanol affect photoreceptor function but do not disturb retinal morphology. Higher levels of methanol exposure cause retinal patterning defects and a small eye phenotype.
Entities:
Keywords:
Zebrafish; cell proliferation; methanol; retina; retinal differentiation; small eye
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