Dmitry José de Santana Sarmento1, Tânia Regina Tozetto-Mendoza2, Laura Masami Sumita2, Ligia Camara Pierroti3, Débora Pallos4, Rubens Caliento1, Michelle Palmieri1, Victor Adriano de Oliveira Martins1, Marina Gallottini1, Claudio Sergio Pannuti2, Paulo Henrique Braz-Silva5,6. 1. Division of Pathology, Department of Stomatology, University of São Paulo School of Dentistry, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, Brazil. 2. Laboratory of Virology, Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470 - Jd. América, São Paulo, SP, Brazil. 3. Department of Infectious Diseases, University of São Paulo School of Medicine, Av. Dr. Arnaldo, 455 - Cerqueira César, São Paulo, SP, Brazil. 4. Department of Dentistry, University of Santo Amaro, Rua Professor Enéas de Siqueira Neto, 340 - Santo Amaro, São Paulo, SP, Brazil. 5. Division of Pathology, Department of Stomatology, University of São Paulo School of Dentistry, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, Brazil. pbraz@usp.br. 6. Laboratory of Virology, Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470 - Jd. América, São Paulo, SP, Brazil. pbraz@usp.br.
Abstract
OBJECTIVE: To describe the shedding profile of human herpesviruses in the saliva of renal transplant recipients. METHODS: This is a prospective case-control study of 50 renal transplant recipients and control group of 50 individuals (non-transplanted and immunocompetent). Mouthwash samples were collected via oral rinse and then submitted to screening for the presence of eight types of herpesviruses by using multiplex PCR. Fisher's exact, chi-square, and Student t tests were used for statistical analysis, and the significance level was set at 5%. RESULTS: The mean age of the study group was 49.42 ± 12.94 years, 28/50 (56%) were female, and the time elapsed after transplantation was 68.20 ± 67.19 months. Herpes simplex virus 1 (HSV-1) (P = 0.025) and Epstein-Barr virus (EBV) (P = 0.024) were, statistically, more excreted in the saliva of renal transplant recipients compared to control group. Gender (P = 1.00) and age (P = 0.563) did not influence the salivary shedding of herpesviruses in renal transplant recipients. Individuals who excreted varicella-zoster virus in saliva had a shorter mean time of transplantation (22:00 + 2.82 months) (P < 0.001). CONCLUSION: Renal transplant recipients excreted herpesviruses more often than controls, especially HSV-1 and EBV, with salivary shedding of herpesviruses being more frequent in patients with recent kidney transplantation. CLINICAL RELEVANCE: The present findings support other longitudinal studies evaluating the relationship between oral shedding of human herpesviruses and clinical presence of active infection and renal transplant failure.
OBJECTIVE: To describe the shedding profile of human herpesviruses in the saliva of renal transplant recipients. METHODS: This is a prospective case-control study of 50 renal transplant recipients and control group of 50 individuals (non-transplanted and immunocompetent). Mouthwash samples were collected via oral rinse and then submitted to screening for the presence of eight types of herpesviruses by using multiplex PCR. Fisher's exact, chi-square, and Student t tests were used for statistical analysis, and the significance level was set at 5%. RESULTS: The mean age of the study group was 49.42 ± 12.94 years, 28/50 (56%) were female, and the time elapsed after transplantation was 68.20 ± 67.19 months. Herpes simplex virus 1 (HSV-1) (P = 0.025) and Epstein-Barr virus (EBV) (P = 0.024) were, statistically, more excreted in the saliva of renal transplant recipients compared to control group. Gender (P = 1.00) and age (P = 0.563) did not influence the salivary shedding of herpesviruses in renal transplant recipients. Individuals who excreted varicella-zoster virus in saliva had a shorter mean time of transplantation (22:00 + 2.82 months) (P < 0.001). CONCLUSION: Renal transplant recipients excreted herpesviruses more often than controls, especially HSV-1 and EBV, with salivary shedding of herpesviruses being more frequent in patients with recent kidney transplantation. CLINICAL RELEVANCE: The present findings support other longitudinal studies evaluating the relationship between oral shedding of human herpesviruses and clinical presence of active infection and renal transplant failure.
Entities:
Keywords:
Human herpesviruses; Kidney transplantation; Oral shedding; Saliva
Authors: Z Mustapic; N Basic-Jukic; P Kes; V Lovcic; Lj Bubic-Filipi; I Mokos; Z Kastelan; S Zekan Journal: Kidney Blood Press Res Date: 2011-06-07 Impact factor: 2.687
Authors: Paulo H Braz-Silva; Karem L Ortega; Nathalie P Rezende; Fabio D Nunes; Marina H C G Magalhães Journal: Diagn Cytopathol Date: 2006-01 Impact factor: 1.582