Chaido Akrivopoulou1, Ingrid M Green2, Nikolaos Donos3, Sean P Nair4, Derren Ready5. 1. Periodontology Unit, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK. 2. Microbiology Unit, Eastman Dental Hospital, UCLH NHS Foundation Trust, 256 Gray's Inn Road, London WC1X 8LD, UK; Department of Microbial Diseases, University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK. Electronic address: i.green@ucl.ac.uk. 3. Periodontology Unit, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK; Centre for Oral Clinical Research, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Turner Street, London E1 2AD, UK. 4. Department of Microbial Diseases, University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK. 5. Department of Microbial Diseases, University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK; Public Health England, Colindale, London NW9 5EQ, UK.
Abstract
OBJECTIVES: Aggregatibacter actinomycetemcomitans is a recognised pathogen involved in aggressive periodontitis. Seven serotypes of A. actinomycetemcomitans exist with a range of virulence and distribution dependent on ethnicity and geography. The ability of A. actinomycetemcomitans to invade soft tissue can necessitate the use of systemic antibiotics for treatment, however variations in its antibiotic susceptibility exist dependent on geographical location. METHODS: Serotypes of A. actinomycetemcomitans isolates from a UK cohort of 50 patients with aggressive periodontitis were determined by PCR. Resistance of the isolates to eight antibiotics [penicillin (1U), amoxicillin (2μg), amoxicillin/clavulanic acid (30μg), metronidazole (5μg), clindamycin (2μg), tetracycline (10μg), ciprofloxacin (5μg) and ceftazidime (30μg)] were determined by disk diffusion according to BSAC guidelines. RESULTS: Prevalences of serotypes a, c, b, e and mixed serotypes were 48%, 22%, 2%, 2% and 12%, respectively. The serotype of isolates from seven patients (14%) could not be deduced by PCR. Of the 56 isolates tested, 100% were resistant to penicillin and metronidazole, 87.5% to clindamycin, 83.9% to amoxicillin and 76.8% to ceftazidime. Low rates of resistance to tetracycline (8.9% resistant) and amoxicillin/clavulanic acid (14.3% resistant) were observed, whereas no isolates were resistant to ciprofloxacin. CONCLUSIONS: As in a number of publications the suggested treatment of aggressive periodontitis includes the combined use of amoxicillin with metronidazole, these results highlight the need for culture and antimicrobial susceptibility investigations in patients with aggressive periodontitis prior to systemic use of antibiotics concomitantly to periodontal therapy.
OBJECTIVES:Aggregatibacter actinomycetemcomitans is a recognised pathogen involved in aggressive periodontitis. Seven serotypes of A. actinomycetemcomitans exist with a range of virulence and distribution dependent on ethnicity and geography. The ability of A. actinomycetemcomitans to invade soft tissue can necessitate the use of systemic antibiotics for treatment, however variations in its antibiotic susceptibility exist dependent on geographical location. METHODS: Serotypes of A. actinomycetemcomitans isolates from a UK cohort of 50 patients with aggressive periodontitis were determined by PCR. Resistance of the isolates to eight antibiotics [penicillin (1U), amoxicillin (2μg), amoxicillin/clavulanic acid (30μg), metronidazole (5μg), clindamycin (2μg), tetracycline (10μg), ciprofloxacin (5μg) and ceftazidime (30μg)] were determined by disk diffusion according to BSAC guidelines. RESULTS: Prevalences of serotypes a, c, b, e and mixed serotypes were 48%, 22%, 2%, 2% and 12%, respectively. The serotype of isolates from seven patients (14%) could not be deduced by PCR. Of the 56 isolates tested, 100% were resistant to penicillin and metronidazole, 87.5% to clindamycin, 83.9% to amoxicillin and 76.8% to ceftazidime. Low rates of resistance to tetracycline (8.9% resistant) and amoxicillin/clavulanic acid (14.3% resistant) were observed, whereas no isolates were resistant to ciprofloxacin. CONCLUSIONS: As in a number of publications the suggested treatment of aggressive periodontitis includes the combined use of amoxicillin with metronidazole, these results highlight the need for culture and antimicrobial susceptibility investigations in patients with aggressive periodontitis prior to systemic use of antibiotics concomitantly to periodontal therapy.
Authors: Oum Keltoum Ennibi; Rolf Claesson; Sanae Akkaoui; Sarah Reddahi; Francis Kwamin; Dorte Haubek; Anders Johansson Journal: Clin Exp Dent Res Date: 2019-01-24
Authors: Yanyan Fu; Sandra Maaβ; Marines du Teil Espina; Anouk H G Wolters; Yanan Gong; Anne de Jong; Erwin Raangs; Girbe Buist; Johanna Westra; Dörte Becher; Jan Maarten van Dijl Journal: mSystems Date: 2022-06-13 Impact factor: 7.324