Climbing and parallel fiber responses recorded intracellularly from Purkinje cell dendrites in guinea pig cerebellar slices.

Climbing fiber responses (CFRs) evoked by white matter stimulation and parallel fiber responses (PFRs) elicited by the stimulation of the surface of the folium were compared using intracellular recording from the dendrites of Purkinje cells in the in vitro slice preparation of the guinea pig cerebellum. Intracellular injection of caesium ions allowed Purkinje cell dendrites to depolarize to a range of -20 to +30 mV, and to reverse both CFR and PFR. The mean reversal potential for the CFR was less negative than for the PFR. CF exhibited a depleting property of a neurotransmitter. The contribution of the Ca-component at the rising phase of CFR was discussed.