Tyler J Loftus1, Andrew J Thomson2, Kolenkode B Kannan3, Ines G Alamo4, Jessica K Millar5, Jessica M Plazas6, Elizabeth E Whitley7, Philip A Efron8, Alicia M Mohr9. 1. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Tyler.Loftus@surgery.ufl.edu. 2. University of Florida, College of Medicine, Gainesville, FL, USA. Electronic address: ajthom09@ufl.edu. 3. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Kolenkode.Kannan@surgery.ufl.edu. 4. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Ines.Alamo@surgery.ufl.edu. 5. University of Florida, College of Medicine, Gainesville, FL, USA. Electronic address: jmillar15@ufl.edu. 6. University of Florida, Gainesville, FL, USA. Electronic address: jessicamplazas@ufl.edu. 7. Pathogenesis, LLC, Gainesville, FL, USA. Electronic address: ewhitley@ufl.edu. 8. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Philip.Efron@surgery.ufl.edu. 9. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Alicia.Mohr@surgery.ufl.edu.
Abstract
BACKGROUND: We hypothesized that clonidine and propranolol would increase VEGF and VEGF-receptor expression and promote lung healing following severe trauma and chronic stress. METHODS: Sprague-Dawley rats were subjected to lung contusion (LC), lung contusion/hemorrhagic shock (LCHS), or lung contusion/hemorrhagic shock/daily restraint stress (LCHS/CS). Clonidine and propranolol were administered daily. On day seven, lung VEGF, VEGFR-1, VEGFR-2, and HMGB1 were assessed by PCR. Lung injury was assessed by light microscopy (*p < 0.05). RESULTS: Clonidine increased VEGF expression following LCHS (43%*) and LCHS/CS (46%*). Clonidine increased VEGFR-1 and R-2 expression following LCHS/CS (203%* and 47%*, respectively). Clonidine decreased HMGB1 and TNF-alpha expression following LCHS/CS (22%* and 58%*, respectively.) Clonidine decreased inflammatory cell infiltration and total Lung Injury Score following LCHS/CS. Propranolol minimally affected VEGF and did not improve lung healing. CONCLUSIONS: Clonidine increased VEGF and VEGF-receptor expression, decreased HMGB1 expression, decreased lung inflammation, and improved lung tissue repair.
BACKGROUND: We hypothesized that clonidine and propranolol would increase VEGF and VEGF-receptor expression and promote lung healing following severe trauma and chronic stress. METHODS:Sprague-Dawley rats were subjected to lung contusion (LC), lung contusion/hemorrhagic shock (LCHS), or lung contusion/hemorrhagic shock/daily restraint stress (LCHS/CS). Clonidine and propranolol were administered daily. On day seven, lung VEGF, VEGFR-1, VEGFR-2, and HMGB1 were assessed by PCR. Lung injury was assessed by light microscopy (*p < 0.05). RESULTS:Clonidine increased VEGF expression following LCHS (43%*) and LCHS/CS (46%*). Clonidine increased VEGFR-1 and R-2 expression following LCHS/CS (203%* and 47%*, respectively). Clonidine decreased HMGB1 and TNF-alpha expression following LCHS/CS (22%* and 58%*, respectively.) Clonidine decreased inflammatory cell infiltration and total Lung Injury Score following LCHS/CS. Propranolol minimally affected VEGF and did not improve lung healing. CONCLUSIONS:Clonidine increased VEGF and VEGF-receptor expression, decreased HMGB1 expression, decreased lung inflammation, and improved lung tissue repair.
Authors: Letitia E Bible; Latha V Pasupuleti; Amy V Gore; Ziad C Sifri; Kolenkode B Kannan; Alicia M Mohr Journal: Surgery Date: 2015-07-21 Impact factor: 3.982
Authors: Tyler J Loftus; Andrew J Thomson; Kolenkode B Kannan; Ines G Alamo; Harry N Ramos; Elizabeth E Whitley; Philip A Efron; Alicia M Mohr Journal: J Surg Res Date: 2016-11-02 Impact factor: 2.192
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