Mingzhen Zhang1, Xiaoyu Wang2, Moon Kwon Han1, James F Collins2, Didier Merlin1,3. 1. Institute for Biomedical Sciences, Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30302, USA. 2. Food Science & Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA. 3. Alanta Veterans Affairs Medical Center, Decatur, GA 30033, USA.
Abstract
AIM: To develop novel siRNA delivery system overcoming the limitations of synthetic nanoparticles, such as potential side effects, nonspecificity and economic production for ulcerative colitis therapy. MATERIALS & METHODS: Nanoparticles composed of edible ginger-derived lipid, termed ginger-derived lipid vehicles (GDLVs) were generated from ginger lipids through hydration of a lipid film, a commonly used method for a liposome fabrication. The morphology, biocompatibility and transfection efficiency of GDLVs loaded with siRNA-CD98 (siRNA-CD98/GDLVs) were characterized by standard methods. RESULTS: Orally administered siRNA-CD98/GDLVs were effectively targeted specifically to colon tissues, resulting in reduced expression of CD98. CONCLUSION: These GDLVs have great promise as efficient siRNA-delivery vehicles while potentially obviating issues related to the traditional synthetic nanoparticles. As such, they help shift the current paradigm of siRNA delivery away from artificially synthesized nanoparticles toward the use of naturally derived nanovehicles from edible plants.
AIM: To develop novel siRNA delivery system overcoming the limitations of synthetic nanoparticles, such as potential side effects, nonspecificity and economic production for ulcerative colitis therapy. MATERIALS & METHODS: Nanoparticles composed of edible ginger-derivedlipid, termed ginger-derivedlipid vehicles (GDLVs) were generated from gingerlipids through hydration of a lipid film, a commonly used method for a liposome fabrication. The morphology, biocompatibility and transfection efficiency of GDLVs loaded with siRNA-CD98 (siRNA-CD98/GDLVs) were characterized by standard methods. RESULTS: Orally administered siRNA-CD98/GDLVs were effectively targeted specifically to colon tissues, resulting in reduced expression of CD98. CONCLUSION: These GDLVs have great promise as efficient siRNA-delivery vehicles while potentially obviating issues related to the traditional synthetic nanoparticles. As such, they help shift the current paradigm of siRNA delivery away from artificially synthesized nanoparticles toward the use of naturally derived nanovehicles from edible plants.
Authors: Michelle M Rietbergen; Sanne R Martens-de Kemp; Elisabeth Bloemena; Birgit I Witte; Arjen Brink; Robert J Baatenburg de Jong; C René Leemans; Boudewijn J M Braakhuis; Ruud H Brakenhoff Journal: Eur J Cancer Date: 2013-12-04 Impact factor: 9.162