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Literature DB >> 2866070

Simultaneous analysis of cell surface antigens, bromodeoxyuridine incorporation and DNA content.

Abstract

A method has been developed for correlating the presence of cell surface markers with bromodeoxyuridine (BrdUrd) uptake. We have found that paraformaldehyde fixation will maintain immunofluorescent cell surface staining through acid denaturation that is necessary for anti-BrdUrd reactivity to single-stranded DNA. In addition, the forward angle light scattering was maintained, even though the cells had been exposed to 2N HCl and detergent. The protocol was tested on three model systems: mouse thymus, a human cell line (CCRF-SB), and peripheral blood leukocytes. It was found that there was no specific loss of lymphocyte subsets.

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Year: 1985 PMID： 2866070 DOI： 10.1002/cyto.990060607

Source DB: PubMed Journal: Cytometry ISSN： 0196-4763

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Cited

13 in total

1. Bromodeoxyuridine (BrdU) immunocytochemistry by exonuclease III (Exo III) digestion.

Authors: W N Dinjens; J ten Kate; M H Lenders; E P van der Linden; F T Bosman
Journal: Histochemistry Date: 1992-10

2. An analysis of the growth and differentiation of B cells isolated from follicles of the ileal Peyer's patch of sheep.

Authors: P J Griebel; W C Davis; J D Reynolds
Journal: Immunology Date: 1992-04 Impact factor: 7.397

3. Advances in cell kinetics.

Authors: D A Rew; G D Wilson
Journal: BMJ Date: 1991-09-07

4. The superantigen Staphylococcus enterotoxin B induces a strong and accelerated secondary T-cell response rather than anergy.

Authors: H Schultz; A Geiselhart; G Sappler; D Niethammer; M K Hoffmann; G E Dannecker
Journal: Immunology Date: 1996-01 Impact factor: 7.397

10. Choice of fixation and denaturation for the triple labelling of intra-cytoplasmic antigen, bromodeoxyuridine and DNA. Application to bone marrow plasma cells.

Authors: M Ffrench; F Morel; C Souchier; M Benchaib; R Catallo; P A Bryon
Journal: Histochemistry Date: 1994-06

Simultaneous analysis of cell surface antigens, bromodeoxyuridine incorporation and DNA content.

1. Bromodeoxyuridine (BrdU) immunocytochemistry by exonuclease III (Exo III) digestion.

2. An analysis of the growth and differentiation of B cells isolated from follicles of the ileal Peyer's patch of sheep.

3. Advances in cell kinetics.

4. The superantigen Staphylococcus enterotoxin B induces a strong and accelerated secondary T-cell response rather than anergy.

5. Improved bromodeoxyuridine/DNA analysis by anti-BudR monoclonal antibody versus right angle light scatter.

6. Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation.

7. Analysis of cell kinetics using a cell division marker: mathematical modeling of experimental data.

8. Transient analysis for antiproliferative gene activity.

9. DNA cell cycle studies in uveal melanoma.

10. Choice of fixation and denaturation for the triple labelling of intra-cytoplasmic antigen, bromodeoxyuridine and DNA. Application to bone marrow plasma cells.