Literature DB >> 28660233

Chase Assay of Protein Stability in Haloferax volcanii.

Xian Fu1, Julie A Maupin-Furlow1,2.   

Abstract

Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study proteolysis pathways in cells. Here, we describe a chase assay in Haloferax volcanii, a halophilic archaeon as the model organism.

Entities:  

Keywords:  Archaea; Chase assay; SAMP; Targeted proteolysis; Ubiquitin-proteasome system

Year:  2017        PMID： 28660233      PMCID： PMC5484168     

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

Review 1.  The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.

Authors:  Michael H Glickman; Aaron Ciechanover
Journal:  Physiol Rev       Date:  2002-04       Impact factor: 37.312

2.  Determining protein half-lives.

Authors:  Pengbo Zhou
Journal:  Methods Mol Biol       Date:  2004

Review 3.  Prokaryotic ubiquitin-like protein modification.

Authors:  Julie A Maupin-Furlow
Journal:  Annu Rev Microbiol       Date:  2014-05-29       Impact factor: 15.500

4.  Involvement of a eukaryotic-like ubiquitin-related modifier in the proteasome pathway of the archaeon Sulfolobus acidocaldarius.

Authors:  Rana S Anjum; Sian M Bray; John K Blackwood; Mairi L Kilkenny; Matthew A Coelho; Benjamin M Foster; Shurong Li; Julie A Howard; Luca Pellegrini; Sonja-Verena Albers; Michael J Deery; Nicholas P Robinson
Journal:  Nat Commun       Date:  2015-09-08       Impact factor: 14.919

5.  Ubiquitin-Like Proteasome System Represents a Eukaryotic-Like Pathway for Targeted Proteolysis in Archaea.

Authors:  Xian Fu; Rui Liu; Iona Sanchez; Cecilia Silva-Sanchez; Nathaniel L Hepowit; Shiyun Cao; Sixue Chen; Julie Maupin-Furlow
Journal:  MBio       Date:  2016-05-17       Impact factor: 7.867
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.