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Literature DB >> 28656583

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.

Steven Skolasinski¹, Angela Panoskaltsis-Mortari².

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Entities: Chemical Disease Gene Species

Keywords: Decellularization; Lung; Negative pressure bioreactor; Organ scaffold

Mesh：

Substances：

Year: 2018 PMID： 28656583 PMCID： PMC5895527 DOI： 10.1007/7651_2017_32

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

9 in total

1. Giving new life to old lungs: methods to produce and assess whole human paediatric bioengineered lungs.

Authors: Joan E Nichols; Saverio La Francesca; Stephanie P Vega; Jean A Niles; Lissenya B Argueta; Michael Riddle; Jason Sakamoto; Grace Vargas; Rahul Pal; Lee Woodson; Jessica Rhudy; Dan Lee; David Seanor; Gerald Campbell; Vicki Schnadig; Joaquin Cortiella
Journal: J Tissue Eng Regen Med Date: 2016-01-12 Impact factor: 3.963

2. Resident cellular components of the human lung: current knowledge and goals for research on cell phenotyping and function.

Authors: Teri J Franks; Thomas V Colby; William D Travis; Rubin M Tuder; Herbert Y Reynolds; Arnold R Brody; Wellington V Cardoso; Ronald G Crystal; Christopher J Drake; John Engelhardt; Maria Frid; Erica Herzog; Robert Mason; Sem H Phan; Scott H Randell; Mary C Rose; Troy Stevens; Julie Serge; Mary E Sunday; Judith A Voynow; Brant M Weinstein; Jeffrey Whitsett; Mary C Williams
Journal: Proc Am Thorac Soc Date: 2008-09-15

1 in total

1. Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries.

Authors: Ashraf Hassanpour; Tahereh Talaei-Khozani; Elias Kargar-Abarghouei; Vahid Razban; Zahra Vojdani
Journal: Stem Cell Res Ther Date: 2018-09-26 Impact factor: 6.832

1 in total

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.

1. Giving new life to old lungs: methods to produce and assess whole human paediatric bioengineered lungs.

2. Resident cellular components of the human lung: current knowledge and goals for research on cell phenotyping and function.

3. Development of a decellularized lung bioreactor system for bioengineering the lung: the matrix reloaded.

4. Automated decellularization of intact, human-sized lungs for tissue engineering.

5. Distribution of lung cell numbers and volumes between alveolar and nonalveolar tissue.

Review 6. Comparison of methods for whole-organ decellularization in tissue engineering of bioartificial organs.

7. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture.

8. Biomimetic Culture Reactor for Whole-Lung Engineering.

9. Ventilation-Based Decellularization System of the Lung.

1. Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries.