Vlad Bloancă1, Amalia Raluca Ceauşu2, Andreea Adriana Jitariu2, Ariana Barmayoun3, Raluca Moş3, Zorin Crăiniceanu4, Tiberiu Bratu1. 1. Department of Plastic Surgery, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania. 2. Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania. 3. Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania. 4. Department of Plastic Surgery, Victor Babes University of Medicine and Pharmacy, Timişoara, Romania zcrainiceanu@gmail.com.
Abstract
AIM: The aim of the study was to assess the effect of autologous fat graft on nerve regeneration by means of immunohistochemistry. MATERIALS AND METHODS: The rat sciatic nerve was used; complete transection followed by primary neurorrhaphy was performed on both hind legs, on the left side a processed fat graft was applied, surrounding the nerve. Nerve biopsies were collected and immunohistochemical procedures were performed for glial fibrillary acidic protein (GFAP) and for neurofilament-associated protein(NFAP). RESULTS: At 4 weeks, GFAP-positive cells were observed in the connective tissue formed between the two nerve endings on the left side only. At 10 weeks, GFAP-positive structures were present and exhibited a tendency to become linear on both sides, with an increased density on the left. NFAP-positive expression was present in the left treated limb with a disorganized pattern. CONCLUSION: Adipose tissue led to the stimulation of GFAP-positive Schwann cells, which could have a positive impact on nerve regeneration in the clinical setting. Copyright
AIM: The aim of the study was to assess the effect of autologous fat graft on nerve regeneration by means of immunohistochemistry. MATERIALS AND METHODS: The rat sciatic nerve was used; complete transection followed by primary neurorrhaphy was performed on both hind legs, on the left side a processed fat graft was applied, surrounding the nerve. Nerve biopsies were collected and immunohistochemical procedures were performed for glial fibrillary acidic protein (GFAP) and for neurofilament-associated protein(NFAP). RESULTS: At 4 weeks, GFAP-positive cells were observed in the connective tissue formed between the two nerve endings on the left side only. At 10 weeks, GFAP-positive structures were present and exhibited a tendency to become linear on both sides, with an increased density on the left. NFAP-positive expression was present in the left treated limb with a disorganized pattern. CONCLUSION: Adipose tissue led to the stimulation of GFAP-positive Schwann cells, which could have a positive impact on nerve regeneration in the clinical setting. Copyright
Authors: André L Bombeiro; Júlio C Santini; Rodolfo Thomé; Elisângela R L Ferreira; Sérgio L O Nunes; Bárbara M Moreira; Ivan J M Bonet; Cesar R Sartori; Liana Verinaud; Alexandre L R Oliveira Journal: Front Cell Neurosci Date: 2016-06-14 Impact factor: 5.505