Xiaoping Xu1, Xiaohua Li1, Lei Zhang1, Zhaohui Liu1, Yuan Pan1, Dong Chen1, Donghua Bin2, Qun Deng3, Y U Sun4, Robert M Hoffman5,6, Zhijian Yang4,5, Hong Yuan7. 1. Department of Anorectal Surgery, Yuhang District First People's Hospital, Hangzhou, P.R. China. 2. Department of Anorectal Surgery, Affiliated First Hospital of Hunan University of Traditional Chinese Medicine, Changsha, P.R. China. 3. Department of Oncological Surgery, Affiliated Second Hospital of Medical College of Zhejiang University, Hangzhou, P.R. China. 4. Origin Biosciences Inc., Nanjing, P.R. China. 5. AntiCancer, Inc., San Diego, CA, U.S.A. 6. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. 7. Department of Cardiology, Yuhang District First People's Hospital, Hangzhou, P.R. China 15799621@qq.com.
Abstract
BACKGROUND/AIM: Hemorrhoidectomy is often associated with significant postoperative complications that may result in slow wound healing. The traditional Chinese medicine (TCM) compound Sophora flavescens (CSF) has shown efficacy on many inflammatory disorders. The aim of the present study was to examine the efficacy of CSF on wound healing in a rat model of perianal ulceration. MATERIALS AND METHODS: A rat model of perianal ulceration was induced by subcutaneous injection of 75% glacial acetic acid. The animals with induced perianal ulcer received topical treatment of low, medium, and high doses of CFS twice daily. Potassium permanganate (PP); 0.02%) was given to the animals for comparison. Macroscopic and histological assessments of the ulcerated area were performed after treatment. The expression of pro-inflammatory cytokines prostaglandin E2 (PGE2) and interleukin-8 (IL-8) was detected by immunohistochemical analysis. RESULTS: Topical administration of medium- and high-dose CSF significantly enhanced perianal ulcer healing as compared to the untreated control (p<0.05). The macroscopic ulceration score was significantly reduced only in the high-dose CSF-treated group as compared to the control (p<0.01). All doses of CSF and PP ameliorated histological damages in the rats with induced perianal ulceration. High-dose CSF or PP significantly reduced the expression of PGE2 and IL-8 as compared to the control (p<0.01). No treatment-related toxicity was found in either the CSF- or the PP-treated mice. CONCLUSION: CSF enhances wound healing in a rat model of perianal ulceration. The inhibitory effect of CSF on pro-inflammatory cytokines PGE2 and IL-8 may be involved in the mechanism of enhanced wound-healing. Copyright
BACKGROUND/AIM: Hemorrhoidectomy is often associated with significant postoperative complications that may result in slow wound healing. The traditional Chinese medicine (TCM) compound Sophora flavescens (CSF) has shown efficacy on many inflammatory disorders. The aim of the present study was to examine the efficacy of CSF on wound healing in a rat model of perianal ulceration. MATERIALS AND METHODS: A rat model of perianal ulceration was induced by subcutaneous injection of 75% glacial acetic acid. The animals with induced perianal ulcer received topical treatment of low, medium, and high doses of CFS twice daily. Potassium permanganate (PP); 0.02%) was given to the animals for comparison. Macroscopic and histological assessments of the ulcerated area were performed after treatment. The expression of pro-inflammatory cytokines prostaglandin E2 (PGE2) and interleukin-8 (IL-8) was detected by immunohistochemical analysis. RESULTS: Topical administration of medium- and high-dose CSF significantly enhanced perianal ulcer healing as compared to the untreated control (p<0.05). The macroscopic ulceration score was significantly reduced only in the high-dose CSF-treated group as compared to the control (p<0.01). All doses of CSF and PP ameliorated histological damages in the rats with induced perianal ulceration. High-dose CSF or PP significantly reduced the expression of PGE2 and IL-8 as compared to the control (p<0.01). No treatment-related toxicity was found in either the CSF- or the PP-treated mice. CONCLUSION: CSF enhances wound healing in a rat model of perianal ulceration. The inhibitory effect of CSF on pro-inflammatory cytokines PGE2 and IL-8 may be involved in the mechanism of enhanced wound-healing. Copyright
Authors: Stefan Riss; Friedrich Anton Weiser; Katrin Schwameis; Thomas Riss; Martina Mittlböck; Gottfried Steiner; Anton Stift Journal: Int J Colorectal Dis Date: 2011-09-20 Impact factor: 2.571
Authors: David E Rivadeneira; Scott R Steele; Charles Ternent; Sridhar Chalasani; W Donald Buie; Janice L Rafferty Journal: Dis Colon Rectum Date: 2011-09 Impact factor: 4.585