Improving trehalose synthase activity by adding the C-terminal domain of trehalose synthase from Thermus thermophilus.

The aim of this work was to study the activities of four other TreS enzymes from different sources linked with or without TtTreS-C. The results showed that a flexible linker peptide between TreS enzymes and TtTreS-C is essential for their activity enhancement. Moreover, the specific activities of the four enzymes were also improved by linking to the TtTreS-C fragment. Together, our study provides novel insights into the functions of the C-terminal domain of TtTreS, and would facilitate its future application in enzyme engineering.