In vivo voltammetry: promise and perspective.

Dopamine, 5-hydroxytryptamine and noradrenaline are electroactive (oxidisable) neurotransmitters in the mammalian brain. Voltammetry, a technique which can measure the concentration of such compounds by their oxidation at an inert electrode, has been applied in vivo in the hope of measuring the release of these neurotransmitters without recourse to perfusion-based or post-mortem analyses. The measurement of neurotransmitter release is, however, complicated by the presence of high concentrations of other electroactive species (ascorbic and uric acids). Nevertheless, when used properly, with due emphasis on pharmacological identification of electrochemical signals, the technique can measure catechol and indole metabolites in vivo. Under certain circumstances the release of the catecholamines and 5-hydroxytryptamine themselves can be measured. The advantages and drawbacks of the voltammetric methodology are discussed.