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Erratum to: Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system.

Bin Lai1,2, Shiqin Yu1,2, Paul V Bernhardt3, Korneel Rabaey4, Bernardino Virdis1,2, Jens O Krömer1,2.   

Abstract

[This corrects the article DOI: 10.1186/s13068-016-0452-y.].

Entities:  

Year:  2017        PMID: 28649274      PMCID: PMC5477287          DOI: 10.1186/s13068-017-0843-8

Source DB:  PubMed          Journal:  Biotechnol Biofuels        ISSN: 1754-6834            Impact factor:   6.040


Erratum to: Biotechnol Biofuels (2016) 9:39 DOI 10.1186/s13068-016-0452-y

After the publication of the article [1], it was brought to our attention that some of the data in Table 2 were incorrect. Please find a correct and updated version of Table 2 in the erratum. Following this Fig. 1 has also been updated; the correct version of Fig. 1 is given in this erratum.
Table 2

Key process parameters of anaerobic glucose conversion of P. putida F1 in the anode compartment of a BES using [Co(bipy)3]3+/2+ or [Fe(CN)6]3−/4− as electron acceptors while poising the anode at +0.697 V vs SHE

[Co(bipy)3]3+/2+ [Fe(CN)6]3−/4−
Carbon balance (%)99.697.6
Coulombic efficiency (%)98.593.3
Yields (molproduct/molglucose)
 Y 2KGA 0.90 ± 0.030.90 ± 0.02
 Y acetic acid 0.073 ± 0.0080.144 ± 0.012
 Y gluconic acid 0.31 ± 0.060.09 ± 0.03
0.25 ± 0.030.09 ± 0.04
 Y electrons 3.94 ± 0.113.88 ± 0.07
Rates (mmol/(gCDW h))
 r glucose −0.26 ± 0.04−0.35 ± 0.07
 r acetic acid 0.019 ± 0.0030.051 ± 0.010
 r 2KGA 0.23 ± 0.040.32 ± 0.06
 r gluconic acid 0.08 ± 0.020.03 ± 0.01
−0.06 ± 0.01−0.03 ± 0.02
 r electrons 1.02 ± 0.181.37 ± 0.26

Data are fitted with linear regression using datasets from ten ([Fe(CN)6]3−/4−) and four ([Co(bipy)3]3+/2+) biological replicates with a total of 79 and 36 samples, respectively (compare Additional file 1: Fig. S3). Carbon balance is calculated from the fitted rates considering carbon content of molecules and assuming equimolar CO2 production when making acetate from glucose. Gluconic acid is a product in the first 100 h and serves as a substrate thereafter, hence 2 yields and rates are given

Fig. 1

Change of biomass (triangles, a), pH (squares, b) and electron production (circles, b) in the anode compartment of a BES reactor of P. putida F1 with K3[Fe(CN)6] as electron acceptor in control (black symbols) and closed circuit with the anode potential poised at +0.697 V (white symbols). Data have been averaged from ten (closed circuit) and three (control) biological replicates with a total of 79 and 30 samples, respectively. Means and standard deviations (X and Y error bars) are given [average sample size n = 7 (closed circuit); exact sample size n = 3 (control)]

Key process parameters of anaerobic glucose conversion of P. putida F1 in the anode compartment of a BES using [Co(bipy)3]3+/2+ or [Fe(CN)6]3−/4− as electron acceptors while poising the anode at +0.697 V vs SHE Data are fitted with linear regression using datasets from ten ([Fe(CN)6]3−/4−) and four ([Co(bipy)3]3+/2+) biological replicates with a total of 79 and 36 samples, respectively (compare Additional file 1: Fig. S3). Carbon balance is calculated from the fitted rates considering carbon content of molecules and assuming equimolar CO2 production when making acetate from glucose. Gluconic acid is a product in the first 100 h and serves as a substrate thereafter, hence 2 yields and rates are given Change of biomass (triangles, a), pH (squares, b) and electron production (circles, b) in the anode compartment of a BES reactor of P. putida F1 with K3[Fe(CN)6] as electron acceptor in control (black symbols) and closed circuit with the anode potential poised at +0.697 V (white symbols). Data have been averaged from ten (closed circuit) and three (control) biological replicates with a total of 79 and 30 samples, respectively. Means and standard deviations (X and Y error bars) are given [average sample size n = 7 (closed circuit); exact sample size n = 3 (control)] Also, during the calculation of specific glucose uptake rate, the authors mistakenly used the unit mmol/L as mmol, and therefore it caused some errors in the calculations of production rate (Table 2) and ATP regeneration rate (Section “Flux balance analysis”—[1]) which need to be corrected. The corrected ATP regeneration rates are 0.02 and 0.38 mmolATP/(gCDW h) for [Co(bpy)3](ClO4)2 from glucose oxidation and membrane-bound ATP synthase respectively, while those numbers for K3[Fe(CN)6] are 0.05 and 0.64 mmolATP/(gCDW h), respectively.
  1 in total

1.  Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system.

Authors:  Bin Lai; Shiqin Yu; Paul V Bernhardt; Korneel Rabaey; Bernardino Virdis; Jens O Krömer
Journal:  Biotechnol Biofuels       Date:  2016-02-18       Impact factor: 6.040

  1 in total

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