| Literature DB >> 28648256 |
Rajeev Ravindran1, Swarna Jaiswal2, Nissreen Abu-Ghannam1, Amit K Jaiswal3.
Abstract
In this study, brewer's spent grain (BSG) was subjected to a range pretreatments to study the effect on reducing sugar yield. Glucose and xylose were found to be the predominant sugars in BSG. Brewers spent grain was high in cellulose (19.21g/100g of BSG) and lignin content (30.84g/100g of BSG). Microwave assisted alkali (MAA) pretreatment was found to be the most effective pretreatment for BSG, where the pretreatment was conducted at 400W for 60s. A maximum reducing yield was observed with high biomass loading (1g/10ml), cellulase (158.76μl/10ml), hemicellulase (153.3μl/10ml), pH (5.4) and an incubation time (120h). Upon enzymatic hydrolysis, MAA pretreated BSG yielded 228.25mg of reducing sugar/g of BSG which was 2.86-fold higher compared to native BSG (79.67mg/g of BSG); simultaneously BSG was de-lignified significantly. The changes in functional groups, crystallinity and thermal behaviour was studies by means of FTIR, XRD and DSC, respectively.Entities:
Keywords: Biomass pretreatment; Brewer’s spent grain; Lignocellulose; Microwave assisted alkali pretreatment; Reducing sugar
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Year: 2017 PMID： 28648256 DOI： 10.1016/j.biortech.2017.06.039
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642