Qiuyue Li1, Huanyu Guan2, Xiaoming Wang2, Yang He2, Hong Sun2, Wenting Tan3, Xiaomei Luo2, Meifeng Su3, Yue Shi4. 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China. 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. 3. College of Traditional Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China. 4. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. Electronic address: shiyue1029@126.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. AIM OF THE STUDY: Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo. Chemical fingerprints of quaternary ammonium alkaloids extracted from Corydalis yanhusuo samples from 37 different sources were identified using UPLC-Q-TOF MS. The protective effects of the 37 samples with respect to H9C2 cell hypoxia-reoxygenation were detected by MTT assays. The fingerprint-efficacy relationship between the chemical fingerprints and cardioprotection afforded by Corydalis yanhusuo was investigated using three chemometric methods. RESULTS: Because of their inherent differences in chemical compositions, the protective effects to H9C2 cell hypoxia-reoxygenation were different. The results of three chemometric methods showed that the source of the Corydalis yanhusuo has an important influence on both the chemical fingerprint and efficacy. In particular, dehydrocorybulbine, 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and palmatine appear to be the main effective components for quality control of this TCM. CONCLUSION: This work provides a general model of combination of UPLC and cardioprotection efficiency to study the fingerprint-efficacy relationship of Corydalis yanhusuo which can offer some references for detecting principal components of Corydalis yanhusuo on cardioprotection efficiency. Fingerprint-efficacy studies also provide a powerful method of quality control in Corydalis yanhusuo and other TCMs.
ETHNOPHARMACOLOGICAL RELEVANCE: Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. AIM OF THE STUDY: Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo. Chemical fingerprints of quaternary ammonium alkaloids extracted from Corydalis yanhusuo samples from 37 different sources were identified using UPLC-Q-TOF MS. The protective effects of the 37 samples with respect to H9C2 cell hypoxia-reoxygenation were detected by MTT assays. The fingerprint-efficacy relationship between the chemical fingerprints and cardioprotection afforded by Corydalis yanhusuo was investigated using three chemometric methods. RESULTS: Because of their inherent differences in chemical compositions, the protective effects to H9C2 cell hypoxia-reoxygenation were different. The results of three chemometric methods showed that the source of the Corydalis yanhusuo has an important influence on both the chemical fingerprint and efficacy. In particular, dehydrocorybulbine, 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and palmatine appear to be the main effective components for quality control of this TCM. CONCLUSION: This work provides a general model of combination of UPLC and cardioprotection efficiency to study the fingerprint-efficacy relationship of Corydalis yanhusuo which can offer some references for detecting principal components of Corydalis yanhusuo on cardioprotection efficiency. Fingerprint-efficacy studies also provide a powerful method of quality control in Corydalis yanhusuo and other TCMs.