Shujun Wan1, Jun Wang2, Jing Wang3, Jia Wu3, Jiaxi Song3, Chen-Yu Zhang4, Chunni Zhang1, Cheng Wang5, Jun-Jun Wang6. 1. Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University, Nanjing 210023, China. 2. Department of Cardiology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China. 3. Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China. 4. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University, Nanjing 210023, China. 5. Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University, Nanjing 210023, China. Electronic address: wangcheng919@smail.nju.edu.cn. 6. Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University, Nanjing 210023, China. Electronic address: wangjunjun9202@163.com.
Abstract
AIMS: To investigate the alteration pattern and physiologic state of islet-specific miR-7 in the serum of patients with type 2 diabetes mellitus (T2DM) and T2DM-associated microvascular complications (T2DMC) and to evaluate its clinical significance. METHODS: The levels of serum miR-7 were firstly examined and compared in 76 T2DM patients, 76 T2DMC patients and 74 age-gender matched controls using RT-qPCR. Subsequently, the physiologic state of serum miR-7 was characterized by determining its concentrations in isolated exosomes and corresponding exosome-free samples from the same three cohorts' samples. Moreover, statistical analyzes were performed to evaluate the associations of serum miR-7 with T2DM and T2DMC. RESULTS: Serum miR-7 was significantly elevated in the T2DM patients [(401.0±34.37) fmol/L, P<0.001] and in the T2DMC patients [(501.4±81.69) fmol/L, P<0.001] when compared with the controls [(175.7±16.59) fmol/L]. Circulating miR-7 was mainly existed as exosome-free form rather than in membrane-bound exosomes. The concentrations of exosome-free miR-7 were markedly higher in the T2DM group [(107.2±9.63) fmol/L, P<0.001] and in the T2DMC group [(122.1±10.80) fmol/L, P<0.001] compared to the control group [(54.18±2.37) fmol/L]. Logistic regression and ROC curve analyses revealed the serum miR-7 was significantly associated with T2DM and microvascular complications (P<0.05). CONCLUSION: Increased serum miR-7 might have the potential as a promising marker for T2DM and its microvascular complications.
AIMS: To investigate the alteration pattern and physiologic state of islet-specific miR-7 in the serum of patients with type 2 diabetes mellitus (T2DM) and T2DM-associated microvascular complications (T2DMC) and to evaluate its clinical significance. METHODS: The levels of serum miR-7 were firstly examined and compared in 76 T2DM patients, 76 T2DMC patients and 74 age-gender matched controls using RT-qPCR. Subsequently, the physiologic state of serum miR-7 was characterized by determining its concentrations in isolated exosomes and corresponding exosome-free samples from the same three cohorts' samples. Moreover, statistical analyzes were performed to evaluate the associations of serum miR-7 with T2DM and T2DMC. RESULTS: Serum miR-7 was significantly elevated in the T2DM patients [(401.0±34.37) fmol/L, P<0.001] and in the T2DMC patients [(501.4±81.69) fmol/L, P<0.001] when compared with the controls [(175.7±16.59) fmol/L]. Circulating miR-7 was mainly existed as exosome-free form rather than in membrane-bound exosomes. The concentrations of exosome-free miR-7 were markedly higher in the T2DM group [(107.2±9.63) fmol/L, P<0.001] and in the T2DMC group [(122.1±10.80) fmol/L, P<0.001] compared to the control group [(54.18±2.37) fmol/L]. Logistic regression and ROC curve analyses revealed the serum miR-7 was significantly associated with T2DM and microvascular complications (P<0.05). CONCLUSION: Increased serum miR-7 might have the potential as a promising marker for T2DM and its microvascular complications.
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