| Literature DB >> 28643259 |
Satoshi Ansai1,2, Masato Kinoshita3.
Abstract
Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.Entities:
Keywords: CRISPR/Cas; Genome editing; Heteroduplex mobility assay; Medaka; Targeted integration; Targeted mutagenesis
Mesh：
Year: 2017 PMID： 28643259 DOI： 10.1007/978-1-4939-7128-2_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745