| Literature DB >> 28643250 |
Taichi Noda1,2, Asami Oji1,2,3, Masahito Ikawa4,5.
Abstract
In mammalian cells, genome editing with the single guide RNA (sgRNA)/Cas9 complex allows for high targeting efficiency within a relatively short time frame with the added benefits of being low cost and easy to design. sgRNA/Cas9-mediated editing in mouse zygotes has accelerated the analysis of gene functions and the generation of mouse models of human diseases. Despite the benefits, this method still suffers from several problems, such as mosaicism in the founder generation which complicates genotyping and phenotypical analyses, and the low efficiency of more complicated genome editing. Thus, we recently established the system for genome editing in embryonic stem (ES) cells and its application for chimeric analysis in mice. In this section, we introduce the procedure for genome editing in mouse zygotes and ES cells.Entities:
Keywords: CRISPR/Cas9; Chimeric analysis; Indel; Knockin; Microinjection; Transfection
Mesh：
Substances：
Year: 2017 PMID： 28643250 DOI： 10.1007/978-1-4939-7128-2_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745