Literature DB >> 28643249

Genome Editing of Mouse by Cytoplasmic Injection.

Takuro Horii1, Izuho Hatada1.   

Abstract

CRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.

Entities:  

Keywords:  CRISPR/Cas; Cytoplast; In vitro transcription; Microinjection; Mouse

Mesh：

Substances：

Year:  2017        PMID： 28643249     DOI： 10.1007/978-1-4939-7128-2_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  The effect of CRISPR constructs microinjection on the expression of developmental genes in Rag1 knocked-out mice embryo.

Authors:  Maryam Salimi; Abolfazl Shirazi; Koushan Sineh Sepehr; Mohsen Norouzian; Vahid Ebrahimi; Maryam Mehravar; Mohammad Majidi; Mohammad M Mehrazar
Journal:  Vet Med Sci       Date:  2021-05-06

Review 2.  Understanding the aetiology and resolution of chronic otitis media from animal and human studies.

Authors:  Mahmood F Bhutta; Ruth B Thornton; Lea-Ann S Kirkham; Joseph E Kerschner; Michael T Cheeseman
Journal:  Dis Model Mech       Date:  2017-11-01       Impact factor: 5.758

3.  Successful generation of epigenetic disease model mice by targeted demethylation of the epigenome.

Authors:  Takuro Horii; Sumiyo Morita; Shinjiro Hino; Mika Kimura; Yuko Hino; Hiroshi Kogo; Mitsuyoshi Nakao; Izuho Hatada
Journal:  Genome Biol       Date:  2020-04-01       Impact factor: 13.583
  3 in total

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