| Literature DB >> 28642138 |
Yuya Okuzaki1, Hidenori Kaneoka2, Ken-Ichi Nishijima1, Seitaro Murakami1, Yuki Ozawa1, Shinji Iijima1.
Abstract
Ten-eleven translocation (TET) methylcytosine dioxygenase has potential as an active eraser to regulate the genomic DNA methylation status. We herein cloned chicken TET (cTET) family genes, and confirmed their functions. Quantitative reverse-transcription PCR showed that cTET1 was strongly expressed in erythrocytes throughout development. This cTET1 expression pattern, together with the results of methylated or hydroxymethylated DNA immunoprecipitation, suggests that cTET1 contributes to demethylation around the promoter region of the definitive-type β-globin gene βΑ in erythroid cells. The knockdown of cTET1 in T2ECs chicken erythroid progenitor cells suppressed the induction of βΑ expression under differentiation conditions. These results suggest that cTET1 plays an important role in erythroid cell differentiation.Entities:
Keywords: 5-Hydroxymethylcytosine (5hmC); Chicken; Erythropoiesis; Ten-eleven translocation (TET); β-globin
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Year: 2017 PMID： 28642138 DOI： 10.1016/j.bbrc.2017.06.113
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575