K Dulasi Durga1, B Adhisivam1, G Vidya2, B Vishnu Bhat1, Zachariah Bobby3, Parkash Chand2. 1. a Department of Neonatology , Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) , Puducherry , India. 2. b Department of Anatomy , Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) , Puducherry , India. 3. c Department of Biochemistry , Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) , Puducherry , India.
Abstract
BACKGROUND: Hyperglycemic mothers have increased oxidative stress during pregnancy, which can adversely affect the outcome in their neonates. OBJECTIVES: To measure the oxidative stress and DNA damage in newborns born to mothers with hyperglycemia and correlate their immediate outcome with the amount of oxidative stress and DNA damage. METHODS: This prospective cohort study conducted in a tertiary care teaching hospital, South India included three groups - newborns born to pregestational diabetic mothers, gestational diabetic mothers, and euglycemic mothers with 24 newborns in each. Oxidative stress parameters - malondialdehyde (MDA), total antioxidant capacity (TAC), and DNA damage (comet assay) were assessed in umbilical cord blood. Association between these parameters and immediate neonatal outcome among three groups were studied. RESULTS: Newborns born to diabetic mothers had more MDA levels and DNA damage compared to euglycemic mothers. There was a positive correlation between comet parameters and MDA levels. There was an inverse correlation between comet parameters and TAC. Newborns born to diabetic mothers were at high risk to develop respiratory distress syndrome, hypoglycemia, and sepsis. CONCLUSIONS: Newborns born to diabetic mothers had increased oxidative stress and DNA damage compared to those born to mothers with euglycemia.
BACKGROUND: Hyperglycemic mothers have increased oxidative stress during pregnancy, which can adversely affect the outcome in their neonates. OBJECTIVES: To measure the oxidative stress and DNA damage in newborns born to mothers with hyperglycemia and correlate their immediate outcome with the amount of oxidative stress and DNA damage. METHODS: This prospective cohort study conducted in a tertiary care teaching hospital, South India included three groups - newborns born to pregestational diabetic mothers, gestational diabetic mothers, and euglycemic mothers with 24 newborns in each. Oxidative stress parameters - malondialdehyde (MDA), total antioxidant capacity (TAC), and DNA damage (comet assay) were assessed in umbilical cord blood. Association between these parameters and immediate neonatal outcome among three groups were studied. RESULTS:Newborns born to diabetic mothers had more MDA levels and DNA damage compared to euglycemic mothers. There was a positive correlation between comet parameters and MDA levels. There was an inverse correlation between comet parameters and TAC. Newborns born to diabetic mothers were at high risk to develop respiratory distress syndrome, hypoglycemia, and sepsis. CONCLUSIONS:Newborns born to diabetic mothers had increased oxidative stress and DNA damage compared to those born to mothers with euglycemia.
Entities:
Keywords:
Comet assay; DNA damage; hyperglycemia; neonatal outcome; oxidative stress
Authors: Naomi Dicks; Karina Gutierrez; Luke Currin; Mariana P de Macedo; Werner G Glanzner; Rafael G Mondadori; Marek Michalak; Luis B Agellon; Vilceu Bordignon Journal: Biol Reprod Date: 2021-07-02 Impact factor: 4.285
Authors: Shayma Ahmed; Ashraf Soliman; Vincenzo De Sanctis; Fawzia Alyafie; Nada Alaaraj; Noor Hamed; Hamdy A Ali; Abdulla Kamal Journal: Acta Biomed Date: 2021-05-12
Authors: Bashar W Badran; Dorothea D Jenkins; Daniel Cook; Sean Thompson; Morgan Dancy; William H DeVries; Georgia Mappin; Philipp Summers; Marom Bikson; Mark S George Journal: Front Hum Neurosci Date: 2020-03-18 Impact factor: 3.169