Sana Jamali1, Mohammed Shahid2, Farrukh Sobia3, Anuradha Singh4, Haris M Khan4. 1. Department of Microbiology, J. N. Medical College and Hospital, A. M. U., Aligarh; Department of Microbiology, Integral Institute of Medical Sciences and Research, Lucknow, Uttar Pradesh, India. 2. Department of Microbiology, J. N. Medical College and Hospital, A. M. U., Aligarh, Uttar Pradesh, India; Department of Microbiology, Immunology and Infectious Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of Bahrain. 3. Department of Microbiology, J. N. Medical College and Hospital, A. M. U., Aligarh, Uttar Pradesh, India; Faculty of Public Health and Tropical Medicine, Jazan University, Kingdom of Saudi Arabia. 4. Department of Microbiology, J. N. Medical College and Hospital, A. M. U., Aligarh, Uttar Pradesh, India.
Abstract
BACKGROUND: Cefotaximases (CTX-M), temoniera (TEM), and sulfhydryl variable (SHV) constitute a rapidly growing cluster of enzymes that have disseminated geographically. They are spreading to species other than Enterobacteriaceae and might be responsible for the presence of blaCTX-M,blaTEM, and blaSHVgenes in Pseudomonas and Acinetobacter spp. The present study was designed to characterize CTX-M, TEM, and SHV phenotypically and genotypically in Pseudomonas and Acinetobacter spp. MATERIALS AND METHODS: A total of 90 isolates (73 Pseudomonas and 17 Acinetobacter spp.), resistant to any of the third-generation cephalosporins, were randomly selected from clinical samples. RESULTS: Of 90 isolates, 64 (71.11%) were tested positive for extended-spectrum β-lactamase (ESBL) production. Among phenotypically tested ESBL producers, forty isolates were randomly selected for molecular characterization. The prevalence of CTX-M, TEM, and SHV was found to be 57.5%, 15%, and 75%, respectively. Multiplex polymerase chain reaction assay categorized blaCTX-Mgenes into Groups 1 and 26 where Group 1 was present in only 5 isolates and Group 25 was present in rest of the 18 isolates. CONCLUSION: This is among the premier systemic reports from India documenting phenotypic and molecular characterization of CTX-M, TEM, and SHV β-lactamases in Pseudomonas and Acinetobacter spp. With judicious use of antibiotics and strict infection control procedures, it may be possible to limit the effects of these newer β-lactamases.
BACKGROUND: Cefotaximases (CTX-M), temoniera (TEM), and sulfhydryl variable (SHV) constitute a rapidly growing cluster of enzymes that have disseminated geographically. They are spreading to species other than Enterobacteriaceae and might be responsible for the presence of blaCTX-M,blaTEM, and blaSHVgenes in Pseudomonas and Acinetobacter spp. The present study was designed to characterize CTX-M, TEM, and SHV phenotypically and genotypically in Pseudomonas and Acinetobacter spp. MATERIALS AND METHODS: A total of 90 isolates (73 Pseudomonas and 17 Acinetobacter spp.), resistant to any of the third-generation cephalosporins, were randomly selected from clinical samples. RESULTS: Of 90 isolates, 64 (71.11%) were tested positive for extended-spectrum β-lactamase (ESBL) production. Among phenotypically tested ESBL producers, forty isolates were randomly selected for molecular characterization. The prevalence of CTX-M, TEM, and SHV was found to be 57.5%, 15%, and 75%, respectively. Multiplex polymerase chain reaction assay categorized blaCTX-Mgenes into Groups 1 and 26 where Group 1 was present in only 5 isolates and Group 25 was present in rest of the 18 isolates. CONCLUSION: This is among the premier systemic reports from India documenting phenotypic and molecular characterization of CTX-M, TEM, and SHV β-lactamases in Pseudomonas and Acinetobacter spp. With judicious use of antibiotics and strict infection control procedures, it may be possible to limit the effects of these newer β-lactamases.
Authors: Dina N Abdelrahman; Aya A Taha; Mazar M Dafaallah; Alaa Abdelgafoor Mohammed; Abdel Rahim M El Hussein; Ahmed I Hashim; Yousif F Hamedelnil; Hisham N Altayb Journal: F1000Res Date: 2020-07-27
Authors: Héctor Sambrano; Julio César Castillo; Carlos W Ramos; Brenda de Mayorga; Olga Chen; Ovidio Durán; Carmelo Ciniglio; Criseida Aguilar; Osvaldo Cisterna; Magaly de Chial Journal: Braz J Infect Dis Date: 2020-12-04 Impact factor: 3.257