| Literature DB >> 28631058 |
Fatemeh Khadivi Derakshan1, Farshad Darvishi2, Mehrouz Dezfulian1, Catherine Madzak3.
Abstract
Glucose oxidase (GOX) is currently used in clinical, pharmaceutical, food and chemical industries. The aim of this study was expression and characterization of Aspergillus niger glucose oxidase gene in the yeast Yarrowia lipolytica. For the first time, the GOX gene of A. niger was successfully expressed in Y. lipolytica using a mono-integrative vector containing strong hybrid promoter and secretion signal. The highest total glucose oxidase activity was 370 U/L after 7 days of cultivation. An innovative method was used to cell wall disruption in current study, and it could be recommended to use for efficiently cell wall disruption of Y. lipolytica. Optimum pH and temperature for recombinant GOX activity were 5.5 and 37 °C, respectively. A single band with a molecular weight of 80 kDa similar to the native and pure form of A. niger GOX was observed for the recombinant GOX in SDS-PAGE analysis. Y. lipolytica is a suitable and efficient eukaryotic expression system to production of recombinant GOX in compered with other yeast expression systems and could be used to production of pure form of GOX for industrial applications.Entities:
Keywords: Aspergillus niger; Characterization; Expression; Glucose oxidase; Yarrowia lipolytica
Mesh：
Substances：
Year: 2017 PMID： 28631058 DOI： 10.1007/s12033-017-0017-8
Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695