| Literature DB >> 28625706 |
M Neeraja1, V Lakshmi2, C Padmasri1, K Padmaja1.
Abstract
The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections.Entities:
Keywords: Gram negative bacilli; Gram stain; Sensitivity; Specificity; Yeast
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Year: 2017 PMID： 28625706 DOI： 10.1016/j.mimet.2017.06.014
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363