Geerte Hoeke1, Kimberly J Nahon2, Leontine E H Bakker3, Sabine S C Norkauer4, Donna L M Dinnes5, Maaike Kockx5, Laeticia Lichtenstein6, Diana Drettwan4, Anne Reifel-Miller7, Tamer Coskun7, Philipp Pagel4, Fred P H T M Romijn8, Christa M Cobbaert8, Ingrid M Jazet3, Laurent O Martinez6, Leonard Kritharides9, Jimmy F P Berbée2, Mariëtte R Boon2, Patrick C N Rensen2. 1. Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: g.hoeke@lumc.nl. 2. Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands. 3. Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands. 4. numares AG, Regensburg, Germany. 5. ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, Australia. 6. Institute of Metabolic and Cardiovascular diseases, I2MC, Inserm, Université de Toulouse, UMR 1048, Toulouse, France. 7. Diabetes/Endocrine Department, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN, USA. 8. Deparment of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands. 9. ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, Australia; Department of Cardiology, Concord Repatriation General Hospital, University of Sydney, Sydney, Australia.
Abstract
BACKGROUND: Cold exposure and β3-adrenergic receptor agonism, which both activate brown adipose tissue, markedly influence lipoprotein metabolism by enhancing lipoprotein lipase-mediated catabolism of triglyceride-rich lipoproteins and increasing plasma high-density lipoprotein (HDL) levels and functionality in mice. However, the effect of short-term cooling on human lipid and lipoprotein metabolism remained largely elusive. OBJECTIVE: The objective was to assess the effect of short-term cooling on the serum lipoprotein profile and HDL functionality in men. METHODS: Body mass index-matched young, lean men were exposed to a personalized cooling protocol for 2 hours. Before and after cooling, serum samples were collected for analysis of lipids and lipoprotein composition by 1H-nuclear magnetic resonance. Adenosine triphosphate-binding cassette A1 (ABCA1)-mediated cholesterol efflux capacity of HDL was measured using [3H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. RESULTS: Short-term cooling increased serum levels of free fatty acids, triglycerides, and cholesterol. Cooling increased the concentration of large very low-density lipoprotein (VLDL) particles accompanied by increased mean size of VLDL particles. In addition, cooling enhanced the concentration of small LDL and small HDL particles as well as the cholesterol levels within these particles. The increase in small HDL was accompanied by increased ABCA1-dependent cholesterol efflux in vitro. CONCLUSIONS: Our data show that short-term cooling increases the concentration of large VLDL particles and increases the generation of small LDL and HDL particles. We interpret that cooling increases VLDL production and turnover, which results in formation of surface remnants that form small HDL particles that attract cellular cholesterol.
BACKGROUND: Cold exposure and β3-adrenergic receptor agonism, which both activate brown adipose tissue, markedly influence lipoprotein metabolism by enhancing lipoprotein lipase-mediated catabolism of triglyceride-rich lipoproteins and increasing plasma high-density lipoprotein (HDL) levels and functionality in mice. However, the effect of short-term cooling on human lipid and lipoprotein metabolism remained largely elusive. OBJECTIVE: The objective was to assess the effect of short-term cooling on the serum lipoprotein profile and HDL functionality in men. METHODS: Body mass index-matched young, lean men were exposed to a personalized cooling protocol for 2 hours. Before and after cooling, serum samples were collected for analysis of lipids and lipoprotein composition by 1H-nuclear magnetic resonance. Adenosine triphosphate-binding cassette A1 (ABCA1)-mediated cholesterol efflux capacity of HDL was measured using [3H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. RESULTS: Short-term cooling increased serum levels of free fatty acids, triglycerides, and cholesterol. Cooling increased the concentration of large very low-density lipoprotein (VLDL) particles accompanied by increased mean size of VLDL particles. In addition, cooling enhanced the concentration of small LDL and small HDL particles as well as the cholesterol levels within these particles. The increase in small HDL was accompanied by increased ABCA1-dependent cholesterol efflux in vitro. CONCLUSIONS: Our data show that short-term cooling increases the concentration of large VLDL particles and increases the generation of small LDL and HDL particles. We interpret that cooling increases VLDL production and turnover, which results in formation of surface remnants that form small HDL particles that attract cellular cholesterol.
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Authors: Christopher R Stephens; Jonathan F Easton; Adriana Robles-Cabrera; Ruben Fossion; Lizbeth de la Cruz; Ricardo Martínez-Tapia; Antonio Barajas-Martínez; Alejandro Hernández-Chávez; Juan Antonio López-Rivera; Ana Leonor Rivera Journal: Front Public Health Date: 2020-05-20