BACKGROUND: Necrotizing enterocolitis (NEC) is a leading cause of morbidity and mortality in premature infants. Increased intestinal permeability is central to NEC development. We have shown that stem cells (SCs) can reduce the incidence and severity of NEC. Our current goal was to investigate the efficacy of four different types of SC in preservation of gut barrier function during NEC. MATERIALS AND METHODS: We compared (1) amniotic fluid-derived mesenchymal SC, (2) bone marrow-derived mesenchymal SC, (3) amniotic fluid-derived neural SC, and (4) enteric neural SC. Premature rat pups received an intraperitoneal injection of 2 × 106 SC or phosphate-buffered saline only and were then subjected to experimental NEC. Control pups were breastfed and not subjected to NEC. After 48 h, animals received a single enteral dose of fluorescein isothiocyanate -labeled dextran (FD70), were sacrificed 4 h later, and serum FD70 concentrations determined. RESULTS: Compared to breastfed, unstressed pups with intact gut barrier function and normal intestinal permeability (serum FD70 concentration 2.22 ± 0.271 μg/mL), untreated pups exposed to NEC had impaired barrier function with significantly increased permeability (18.6 ± 4.25 μg/mL, P = 0.047). Pups exposed to NEC but treated with SC had significantly reduced intestinal permeability: Amniotic fluid-derived mesenchymal SC (9.45 ± 1.36 μg/mL, P = 0.017), bone marrow-derived mesenchymal SC (6.73 ± 2.74 μg/mL, P = 0.049), amniotic fluid-derived neural SC (8.052 ± 1.31 μg/mL, P = 0.0496), and enteric neural SC (6.60 ± 1.46 μg/mL, P = 0.033). CONCLUSIONS: SCs improve gut barrier function in experimental NEC. Although all four types of SC reduce permeability equivalently, SC derived from amniotic fluid may be preferable due to availability at delivery and ease of culture, potentially enhancing clinical translation.
BACKGROUND:Necrotizing enterocolitis (NEC) is a leading cause of morbidity and mortality in premature infants. Increased intestinal permeability is central to NEC development. We have shown that stem cells (SCs) can reduce the incidence and severity of NEC. Our current goal was to investigate the efficacy of four different types of SC in preservation of gut barrier function during NEC. MATERIALS AND METHODS: We compared (1) amniotic fluid-derived mesenchymal SC, (2) bone marrow-derived mesenchymal SC, (3) amniotic fluid-derived neural SC, and (4) enteric neural SC. Premature rat pups received an intraperitoneal injection of 2 × 106 SC or phosphate-buffered saline only and were then subjected to experimental NEC. Control pups were breastfed and not subjected to NEC. After 48 h, animals received a single enteral dose of fluorescein isothiocyanate -labeled dextran (FD70), were sacrificed 4 h later, and serum FD70 concentrations determined. RESULTS: Compared to breastfed, unstressed pups with intact gut barrier function and normal intestinal permeability (serum FD70 concentration 2.22 ± 0.271 μg/mL), untreated pups exposed to NEC had impaired barrier function with significantly increased permeability (18.6 ± 4.25 μg/mL, P = 0.047). Pups exposed to NEC but treated with SC had significantly reduced intestinal permeability: Amniotic fluid-derived mesenchymal SC (9.45 ± 1.36 μg/mL, P = 0.017), bone marrow-derived mesenchymal SC (6.73 ± 2.74 μg/mL, P = 0.049), amniotic fluid-derived neural SC (8.052 ± 1.31 μg/mL, P = 0.0496), and enteric neural SC (6.60 ± 1.46 μg/mL, P = 0.033). CONCLUSIONS: SCs improve gut barrier function in experimental NEC. Although all four types of SC reduce permeability equivalently, SC derived from amniotic fluid may be preferable due to availability at delivery and ease of culture, potentially enhancing clinical translation.
Authors: Cüneyt Tayman; Duygu Uckan; Emine Kilic; Ahmet Tulga Ulus; Alparslan Tonbul; Ibrahim Murat Hirfanoglu; Fatma Helvacioglu; Hacer Haltas; Burhan Koseoglu; M Mansur Tatli Journal: Pediatr Res Date: 2011-11 Impact factor: 3.756
Authors: Natalie A Drucker; Jan P Te Winkel; W Christopher Shelley; Kenneth R Olson; Troy A Markel Journal: J Pediatr Surg Date: 2019-03-01 Impact factor: 2.545
Authors: Chhinder P Sodhi; Raheel Ahmad; Hongpeng Jia; William B Fulton; Carla Lopez; Andres J Gonzalez Salazar; Asuka Ishiyama; Maame Sampah; Steve Steinway; Sanxia Wang; Thomas Prindle; Menghan Wang; David L Steed; Howard Wessel; Ziv Kirshner; Larry R Brown; Peng Lu; David J Hackam Journal: Am J Physiol Gastrointest Liver Physiol Date: 2022-07-12 Impact factor: 4.871
Authors: Bo Li; Carol Lee; Marissa Cadete; Joshua S O'Connell; Mashriq Alganabi; Dorothy Lee; Niloofar Ganji; Hiromu Miyake; Steven R Botts; Kathene C Johnson-Henry; Pekka Maattanen; Philip M Sherman; Agostino Pierro Journal: Pediatr Res Date: 2021-09-24 Impact factor: 3.756
Authors: Bo Li; Carol Lee; Joshua S O'Connell; Lina Antounians; Niloofar Ganji; Mashriq Alganabi; Marissa Cadete; Francesca Nascimben; Yuhki Koike; Alison Hock; Steven R Botts; Richard Y Wu; Hiromu Miyake; Adam Minich; Michael F Maalouf; Elke Zani-Ruttenstock; Yong Chen; Kathene C Johnson-Henry; Paolo De Coppi; Simon Eaton; Pekka Maattanen; Paul Delgado Olguin; Augusto Zani; Philip M Sherman; Agostino Pierro Journal: Cell Death Dis Date: 2020-09-14 Impact factor: 8.469