Alejandra Aquino-Andrade1, Gabriel Martínez-Leyva1, Jocelin Mérida-Vieyra1, Patricia Saltigeral2, Antonino Lara3, Wendy Domínguez4, Silvestre García de la Puente5, Agustín De Colsa6. 1. Molecular Microbiology Laboratory, National Institute of Pediatrics, Mexico City, Mexico. 2. Department of Pediatric Infectious Diseases, National Institute of Pediatrics, Mexico City, Mexico. 3. Bacteriology Laboratory, National Institute of Pediatrics, Mexico City, Mexico. 4. Department of Epidemiology, National Institute of Pediatrics, Mexico City, Mexico. 5. Department of Research Methodology, Instituto Nacional de Pediatría, Mexico City, Mexico. 6. Molecular Microbiology Laboratory, National Institute of Pediatrics, Mexico City, Mexico; Department of Pediatric Infectious Diseases, National Institute of Pediatrics, Mexico City, Mexico. Electronic address: agustin.decolsa@infecto.mx.
Abstract
OBJECTIVES: To evaluate the usefulness of real-time polymerase chain reaction (RT-PCR) as a diagnostic method for the detection of Bordetella pertussis in hospitalized patients aged <1 year with a clinical diagnosis of whooping cough, as well as to identify the role of household contacts as a source of infection. STUDY DESIGN: This was a prospective, multicenter study of infants aged <1 year who were hospitalized with symptoms suggestive of whooping cough. Nasopharyngeal samples were obtained for culture and RT-PCR testing. The clinical and epidemiologic characteristics and outcomes were analyzed. B pertussis detection and symptoms in household contacts of patients diagnosed with pertussis were studied. RESULTS: A total of 286 patients were included; of these, 67.1% had B pertussis and 4.5% had Bordetella spp. Complications occurred in 20% of patients, and the mortality rate was 6.7%. Of 434 contacts studied, 111 were mothers of study infants, representing the most frequently B pertussis-infected group and the main symptomatic contact. CONCLUSION: The use of RT-PCR permits improved detection and diagnosis of pertussis and a better understanding of the epidemiology of sources of infection. The complications and mortality rate of pertussis continue to be high. Household contacts are confirmed as a frequent source of infection of B pertussis in young children.
OBJECTIVES: To evaluate the usefulness of real-time polymerase chain reaction (RT-PCR) as a diagnostic method for the detection of Bordetella pertussis in hospitalized patients aged <1 year with a clinical diagnosis of whooping cough, as well as to identify the role of household contacts as a source of infection. STUDY DESIGN: This was a prospective, multicenter study of infants aged <1 year who were hospitalized with symptoms suggestive of whooping cough. Nasopharyngeal samples were obtained for culture and RT-PCR testing. The clinical and epidemiologic characteristics and outcomes were analyzed. B pertussis detection and symptoms in household contacts of patients diagnosed with pertussis were studied. RESULTS: A total of 286 patients were included; of these, 67.1% had B pertussis and 4.5% had Bordetella spp. Complications occurred in 20% of patients, and the mortality rate was 6.7%. Of 434 contacts studied, 111 were mothers of study infants, representing the most frequently B pertussis-infected group and the main symptomatic contact. CONCLUSION: The use of RT-PCR permits improved detection and diagnosis of pertussis and a better understanding of the epidemiology of sources of infection. The complications and mortality rate of pertussis continue to be high. Household contacts are confirmed as a frequent source of infection of B pertussis in young children.
Authors: Varun K Phadke; John P McCracken; Jennifer L Kriss; Maria R Lopez; Kim A Lindblade; Joe P Bryan; Miguel E Garcia; Carlos E Funes; Saad B Omer Journal: J Pediatric Infect Dis Soc Date: 2018-12-03 Impact factor: 5.235
Authors: Juana Del Valle-Mendoza; Cristina Del Valle-Vargas; Ronald Aquino-Ortega; Luis J Del Valle; Erico Cieza-Mora; Wilmer Silva-Caso; Jorge Bazán-Mayra; Victor Zavaleta-Gavidia; Miguel Angel Aguilar-Luis; Hernán Cornejo-Pacherres; Johanna Martins-Luna; Angela Cornejo-Tapia Journal: Iran J Microbiol Date: 2021-02
Authors: Juana Del Valle-Mendoza; Wilmer Silva-Caso; Miguel Angel Aguilar-Luis; Cristina Del Valle-Vargas; Erico Cieza-Mora; Johanna Martins-Luna; Ronald Aquino-Ortega; Andrea Silva-Vásquez; Jorge Bazán-Mayra; Pablo Weilg Journal: BMC Res Notes Date: 2018-05-18