Enhanced Skin Regeneration by Herbal Extract-Coated Poly-L-Lactic Acid Nanofibrous Scaffold.

The wound healing process is directly related to the type of treatment. Existing methods of treatment are not responsive enough for severe wounds. The aim of this study was the potential capacity investigation of poly-L-lactic acid (PLLA) nanofibrous scaffolds coated by aloe vera gel for wound dressing applications. In this study, electrospinning method was used for preparing PLLA nanofibers, and after characterization by SEM and MTT, its influence on the wound healing process was investigated with and without aloe vera gel as a wound dressing in full-thickness skin defect in mice. Band-Aids were used as a positive control and vaseline gauze as a negative control. SEM and MTT assays confirmed the nanometer size and biocompatibility of fabricated nanofibers. Macroscopic and histopathological characteristics were evaluated at the end of days 7, 12, and 17 and their results showed that the gel-coated scaffold accelerated the wound-healing process compared with other groups. At the end of the experiment, it was shown that during the whole time of study, gel-coated scaffold had the highest overall repair score. Therefore, gel-coated PLLA scaffold would be an ideal construct for wound healing and skin regenerative medicine application.