Adaptive self-assembly and induced-fit transformations of anion-binding metal-organic macrocycles.

Container-molecules are attractive to chemists due to their unique structural characteristics comparable to enzymes and receptors in nature. We report here a family of artificial self-assembled macrocyclic containers that feature induced-fit transformations in response to different anionic guests. Five metal-organic macrocycles with empirical formula of MnL2n (M=Metal; L=Ligand; n=3, 4, 5, 6, 7) are selectively obtained starting from one simple benzimidazole-based ligand and square-planar palladium(II) ions, either by direct anion-adaptive self-assembly or induced-fit transformations. Hydrogen-bonding interactions between the inner surface of the macrocycles and the anionic guests dictate the shape and size of the product. A comprehensive induced-fit transformation map across all the MnL2n species is drawn, with a representative reconstitution process from Pd7L14 to Pd3L6 traced in detail, revealing a gradual ring-shrinking mechanism. We envisage that these macrocyclic molecules with adjustable well-defined hydrogen-bonding pockets will find wide applications in molecular sensing or catalysis.

Coordination-driven self-assembly has become one of the most convenient strategies for the bottom-up construction of functional molecular ensembles12345. On the basis of elaborate symmetry considerations, numerous two-dimensional (2D) and three-dimensional (3D) architectures have been obtained in ease by the simple combination of designed ligands (L) and metal ions (M), which show great potential in the modulation of reactivity and/or photoelectric properties of guest molecules by encapsulation678910. To ensure the directed assembly, rigid ligands are most often employed51112. Flexible ligands, which usually give rise to interconvertible architectures, are far less utilized in coordination-directed self-assembly13. However, assemblies available in nature favour flexibility over rigidity. For example, enzymes are rather flexible structures, the active site of which is continuously reshaped by interactions with specific substrates, following the so called induced-fit mechanism14.

Anion receptor chemistry has witnessed great advances in the past decades. This area of supramolecular chemistry has a number of potential applications in biology, environment and the food industry151617. The fast developing coordination-driven supramolecular chemistry has provided enormous examples of metal-organic assembled systems that can recognize, respond to, or sense negative-charged species1819202122. However, most metal-organic receptors reported so far are of invariant structures, aiming to reach high selectivity towards targeting anions. Adaptive anion receptors, that is, receptors that can continuously transform its shape and size to maximize the binding interactions with different anions, remain elusive23242526. For example, seminal work by Hasenknopf et al.23 has shown that use of flexible tris-bipy ligands and iron(II) lead to the formation of a dynamic combinatorial system272829, where a set of circular helicates is expressed depending on the anions present during the self-assembly process.

Herein, we report an artificial assembly system that features adaptive self-assembly and induced-fit transformation properties in the presence of anionic guests. A family of metal-organic macrocycles with the general formula of PdL2 (n=3, 4, 5, 6, 7) are selectively obtained starting from one simple ligand and square-planar PdII ions. Hydrogen bonding between the inner surface of the macrocycles and the bound guests, different anions in this case, dictates the shape and size of the final product. In situ anion-adaptive self-assembly gives rise to the PdL2 species for n=3, 6, 7. For n=4, 5, post-synthetic transformations263031323334 from other macrocycles are employed, featuring an induced-fit transformation process. Five distinct macrocycles are clearly characterized by NMR, ESI-TOF-MS, and in the case of n=3, 4, 5, 6 by single crystal X-ray diffraction. Moreover, a comprehensive map showing all the transformations across the macrocyclic species was drawn, with a representative reconstitution process from Pd7L14 to Pd3L6 traced in detail by titration experiments, revealing a gradual ring-shrinking mechanism.

Results

Syntheses and characterization of metal-organic macrocycles 2–6

It is well-established that self-assembly of rigid planer bidentate pyridinyl ligands with specific bent angles and square-planar PdII ions will lead to a group of PdL2 molecular spheres3536373839404142. Considering the inherent topological relationships, we propose that macrocyclic complexes may also be obtained when the ligand is nonplanar43. In this study, we choose a very simple nonplanar bidentate ligand (1) and PdII ions as our building blocks (Fig. 1). Ligand 1 has two unique features: first it is not conjugated, so that two benzoimidazole rings are reasonably free to rotate and bent, giving rise to the conformational flexibility of its coordination geometry44; and second benzoimidazole bears an acidic CH bond that can act as hydrogen-bond donor, especially after its coordination to a metal4546. In fact, varieties of anion-binding hosts utilizing the imidazole motif, either of pure organic or metal-organic forms, have been reported47. We envisage that anions with different size and shape will dictate the arrangement of ligand 1 during the self-assembly process, thus providing the driving force for the otherwise complicated system towards thermodynamically preferable outcome.

Figure 1

Self-assembly of anion-binding metal-organic macrocycles.

Self-assembly of anion-binding metalorganic macrocycles with an empirical formula of PdL2n (n = 3, 4, 5, 6, 7). Dashed arrows indicate that Pd4L8 and Pd5L10 were obtained by induced-fit transformation from other macrocycles instead of direct self-assembly.

Reaction of ligand 1 (20.07 μmol) with a half equivalent of Pd(NO3)2 (10.04 μmol) in 1 ml dimethyl sulfoxide ([D6]DMSO) at 70 °C for 5 h leads to the quantitative formation of a single compound, as confirmed by 1H NMR spectroscopy (Fig. 2a,b). Compared with the free ligand, the peak of He on benzimidazole of the assembly was shifted downfield from 8.77 to 9.00 p.p.m., suggesting the loss of electron density on the imidazole ring due to coordination or the involvement of Hydrogen bonding. The diastereotopic environment of the methylene protons, which split into a pair of doublets in a 1:1 ratio, indicates a cis conformation of the ligands in the product44. Diffusion-ordered NMR spectrum (DOSY) (Supplementary Fig. 4) confirmed that all peaks have the same diffusion coefficient, with an estimated diameter of 1.12 nm for the assembly. The composition of product formulated as Pd3L6(NO3)6 (2) was then clearly provided by high-resolution ESI-TOF-MS. Prominent peaks observed at m/z=1028.1468, and 665.1049 correspond to the multiple-charged [2–(NO3−)] (n=2, 3) molecular-ion signals, with consecutive loss of the NO3− counter-ions. Moreover, the isotopic patterns of each resolved peaks were also in good agreement with the simulated values (Fig. 2g).

Figure 2

1H NMR and ESI-TOF-MS spectra.

(a–f) are 1H NMR (400 MHz, [D6]DMSO, 298 K) spectra for free ligand 1 and the complexes 2, 3, 4, 5, 6, respectively; (g–k) are ESI-TOF-MS spectra for complexes 2, 3, 4, 5 and 6 with insets showing the representative observed and calculated isotope patterns.

To our surprise, a clearly distinct product was obtained when Pd(BF4)2 was used during the self-assembly, in a similar reaction condition as described above (See Methods section for details). As shown in Fig. 2e, all the proton signals of the ligand on this complex strongly split into two sets in a 1:1 ratio, with one set of signals obviously downfield shifted and the other upfield shifted (except for the CH on imidazole) with respect to those of the free ligand. Such observation is clearly different from the trinuclear compound 2, where only the protons on the methylene groups are split. This suggests two different chemical environments for the benzoimidazole moities in the final product. On the basis of 1H-1H COSY experiment, all the signals could be fully assigned (Supplementary Fig. 8). DOSY spectrum (Supplementary Fig. 9) reveals the formation of a new product with an estimated diameter of 2.23 nm, which is dramatically larger than that of 2. ESI-TOF-MS (Fig. 2j) discloses that this complex is formulated as Pd6L12·(BF4)12 (5), with prominent peaks observed at m/z=689.7848, 844.9425, 1077.9288, 1466.2397 and 2242.8695, corresponding to [5–(BF4−)] (n=2–6).

On the basis of this unexpected structural switch from Pd3L6 to Pd6L12 by varing the counter ions from NO3− to BF4−, we then postulate that other counter ions with larger size may induce the formation of higher nuclear assembly. Indeed, pure heptanuclear Pd7L14·(PF6)14 (6a) or Pd7L14(OTf)14 (6b) complexes were quantitatively obtained by replacing the metal source with Pd(PF6)2 or Pd(OTf)2, respectively, the composition of which were determined in a similar manner by NMR and ESI-TOF-MS (Fig. 2f,k and Supplementary Figs 13–21). Similar diastereotopic splitting of 1H NMR signals into 1:1 ratio was also observed for compound 6.

Direct synthesis of the missing PdL2 macrocycles between n=3 and 6 were unsuccessful. Instead, the intermediate-sized Pd4L8 and Pd5L10 could be obtained by anion-induced transformation processes. In a typical procedure, tetrabutylammonium hydrogen sulfate (10.27 mg, 30.24 μmol, 4.5 eq.) was added to a 1 ml [D6]DMSO solution of compound 5 (6.72 μmol) and the mixture was heat at 70 °C for 3 h. 1D (Fig. 2c) and 2D NMR (Supplementary Figs 25 and 26) indicated the total transformation of 5 into a new product. In the 1H NMR, the diagnostic diastereomeric splitting of the ligands signals on 5 gradually disappeared with the origin of another single set of signals (except for the methylene protons). Moreover, He on the imidazole rings were significantly shifted downfield from 8.75 p.p.m. and 7.86 p.p.m. to 10.46 p.p.m.), indicating the involvement of strong Hydrogen-bonding interactions with the bisulfate ions. DOSY revealed that the new complex was of a diameter of 1.51 nm, a value in between of the compound 2 and 5. Even with intrinsic mixed counter anions, ESI-TOF-MS spectrum clearly confirmed the formation of the tetranuclear Pd4L8 compound (3), with prominent peaks observed at m/z=650.8434, 896.7930, 1388.6908, corrsponding to [Pd4L8(SO4)2]4+, [Pd4L8(SO4)2(BF4)1]3+, [Pd4L8(SO4)2(BF4)2]2+, respectively. The loss of protons on bisulfates anions was possibly due to the total 8+ charge on the host or the presence of multiple Hydrogen bonding between the host and anions. Under similar conditions, induced-fit transformation from 6 to 3 was also quantitative (see discussion below).

The conversion from M6L12 to the M5L10 structure was beyond anticipation. Our initial thought was to induce the formation of higher-nuclear PdL2 complexes (for n>7), using bulkier counter-ions. However, treating compound 5 (6.72 μmol) with 0.8 eq. tetrabutylammonium heptamolybdate ((t-Bu4N)6Mo7O24, 13.48 mg, 5.38 μmol) in 1 ml [D6]DMSO solution converted 5 into Pd5L10 (4) (same conditions as above). The formation of this new complex was indicated by 1H NMR (Fig. 2d), where all signals become severely broadened with the He significantly split into a complicated pattern, presumably due to a mismatch of symmetry between the host and the guest anion. However, 1H NMR at 100 °C becomes much simplified (Supplementary Fig. 30). In particular, the complicated pattern observed for He coalesced into one single broadened peak with clear upfield-shifting, indicating the break of H bonds and increase of symmetry at elevated temperatures. DOSY revealed that the new complex was of a diameter of 1.84 nm (Supplementary Fig. 34). ESI-TOF-MS reveals that a new pentanuclear Pd5L10 compound (4) with an encapsulated [HMo7O24]5− cluster was formed, with prominent peaks observed at m/z = 814.3625, 1039.7043 and 1414.9399 corresponding to [Pd5L10(HMo7O24)1]5+, [Pd5L10(HMo7O24)1(BF4)1]4+ and [Pd5L10(HMo7O24)(BF4)2]3+, respectively (Fig. 2i).

The structures of the self-assembled PdL2 complexes for n=3, 4, 5, 6 were unambiguously determined by single crystal diffraction studies facilitated by high-power X-ray source at the Shanghai Synchrotron Radiation Facility (SSRF). Crystal structures reveal that all complexes shares a similar donut-shaped structure, where 2n bent bidentate ligands are connected by n square-planar coordinated PdII ions in a cyclic fashion. Ligands can be divided into two layers by an imaginary plane of the metal centres. On the basis of crystal structures, we can clearly see that hydrogen-bonding interactions between the host framework and its entrapped anions played an important role in dictating the size of the macrocycles.

High quality single crystals of compound 2 suitable for crystallographic analysis were obtained by slow vapour diffusion of dichloromethane (DCM) into a DMSO solution of the complex over 3 weeks, which crystallized in a P space group with two macrocycles sitting in the unit cell. This smallest donut-shaped assembly has an out diameter of 1.74 nm and a height of 0.87 nm, which can be regarded as a trimeric structure consisting three Pd2L2 square building blocks by sharing the PdII centres (Fig. 3a,b). Interestingly, three nitrate anions were H bonded inside the cavity, which form a three-layered sandwich conformation. One NO3− is in a perpendicular orientation to the other two parallel ones, possibly due to electrostatic repulsion. Each nitrate is involved in at least six Hydrogen-bonding interactions with the inward CH from the imidazole and methylene groups. We propose these multi-point H bonds act as glues to tie the ligand strands together. Similarly, diffusion of DCM into a DMSO solution of 3 resulted in the crystalization of this tetranuclear species (Fig. 3c,d). Two SO42− are encapsulated inside the cavity in this case, each of which is sextuple H bonded with three ligands on the same layer.

Figure 3

X-ray single crystal structures of compounds 2 and 3.

Top (a,c) and side (b,d) views of the X-ray crystal structures of compounds 2 and 3. (Colour scheme: H bondings, red dashed lines; Pd, yellow; C, black; N, blue; S, pink; O, red; H, green. Solvents and anions located outside the macrocycles are omitted for clarity.)

Crystals for the Pd5L10 complex (4) were also obtained by a similar method described above. The pentagonal topology of the framework was clearly confirmed (Fig. 4a,b). Possibly due to the mismatch of symmetry in this host-guest complex, modelling of the encapsulated HMo7O245− anion with the known connectivity4849 were unsuccessful and its contribution was removed by the SQUEEZE routine50. We propose that the HMo7O245− guest inside the confined cavity is severely disordered or adopting an unusual geometry. It has to be pointed out that the nature of the encapsulated [HMo7O24]5− cluster is unclear at this stage and detailed host-guest chemistry of these macrocycles with polyoxometalates deserves further study. After a long trail-and-error, high quality single crystals of 5 were fortunately obtained by a co-crystallization method with 1.0 eq. of NaB(ArF)4 (ArF=3,5-bis(trifluoromethyl)phenyl). At this condition, 1H or 19F NMR (Supplementary Figs 10 and 11) did not show obvious chemical shift for both the host and the [B(ArF)4]− anion, indicating that there is no strong interaction between them. Compound 5 crystallized in an orthogonal R–3c space group with a huge unite cell volume of 119,056 Å3 (Fig. 4c,d). The dimension of this self-assembled macrocycle spans an out diameter of 2.24 nm and inner diameter of 1.19 nm. Compared to the symmetrical Pd2L2 squares units existing on the crystal structures of 2, 3 and 4, the six Pd2L2 units on 5 were severely distorted into a rectangular conformation, which provides a good reason for the split signals observed in its 1H NMR. Such structural distortion also reflects the flexibility of ligand 1. Due to the low-diffraction nature of this sample, only the Pd6L12 connectivity was confirmed and no detailed anion binding mode could be modelled. In crystal packing, two giant channels are present along the c axis due to layer-by-layer stacking of the ring-shaped complexes, one of which is defined by the ring cavity with a diameter of 1.19 nm, the other is 1.75 nm encircled by six adjacent molecules, where we hypothesize is filled with the highly disordered bulky [B(ArF)4]− anions. Many attempts to crystallize the heptanuclear 6 were unsuccessful, possibly due to its low symmetry and big size. This structure was then modelled by molecular mechanical optimizations and depicted in Fig. 4e,f.

Figure 4

X-ray single crystal structures of compounds 4, 5 and simulated structures of complex 6.

Top (a,c) and side (b,d) views for the X-ray crystal structures of complexes 4 and 5; (e,f) are similar views for the simulated structure of complex 6. (Same colour schemes were used as for Fig. 3.)

Discussion

Taking advantage of the anion-adaptivity observed during the self-assembly, we then managed to draw a comprehensive map detailing the induced-fit transformation processes between all species of the family (Fig. 5a and Supplementary Figs 42–58). First of all, we found that addition of 30 eq. of KNO3 to the Pd7L14 complex (PF6− salt) or Pd6L12 complex 5 resulted in the quantitative transformation to Pd3L6. The (Mo7O24)@Pd5L10 was found to be the the most stable host-guest complex for this dynamic combinatorial system, which could be quantitatively obtained by additon of (t-Bu4N)6Mo7O24 to either compounds 2, 3, 5 or 6. In fact, this host-guest complex is so stable that once formed it will never transform into other macrocycles. Similarly, Pd3L6 could be quantitatively obtained by the templation effect of the NO3− anion starting from Pd4L8, Pd6L12 or Pd7L14. SO42−, on the contrary, is a weaker template comparing to NO3−, possibly due to the fact that Pd3L6 can encapsulate three NO3− while Pd4L8 can only host two SO42−. Meanwhile, transformation from Pd7L14 to Pd6L12 was obtained in only 80% yield even if 35 eq. of BF4− anion was used, suggesting only a subtle energy difference between them. On the basis of this map, a sequence of templating-effect like Mo7O246−>NO3−>SO42−>BF4−>PF6−=OTf− was obtained for this dynamic combinatorial system.

Figure 5

Induce-fit transformations between macrocycles.

(a) Cross map showing all the transformations between the macrocyclic species (conditions for a: NO3− ; b: HSO4−; c: Mo7O246−; d: BF4−); (b) 1H NMR (400 MHz, [D6]DMSO, 298 K) titration spectra showing the gradual transformation from the heptanuclear complex 6a to the trinuclear complex 2 by sequential addition of KNO3 (bottom up: 0, 1, 2, 4, 10, 30 eq.) with He signals highlighted for Pd3L6: red, Pd4L8: sky blue, Pd5L10: green, Pd6L12: pink and Pd7L14: dark blue, respectively; (c) Composition distributions for all the PdL2 species obtained by the integrals of the He signals from the titration experiment.

Finally, a detailed tranformation process from Pd7L14 (6a) to Pd3L6 was traced by titration experiments following the sequential addition of NO3− (Fig. 5b,c). 1H NMR and ESI-TOF-MS showed that the transformation took place via a gradual shrinking of the macrocyclic framework. On the basis of a careful assignment of the NMR and ESI-TOF-MS signals (Supplementary Figs 59–70 and Supplementary Tables 5 and 6), it was found that after adding 1 eq. of NO3− ([NO3−]:[PF6−]=1:14) the Pd7L14 macrocycle starts to transform into Pd6L12 and Pd4L8, whose contents reach maxima when 3 eq. of NO3− were added. Meanwhile, formation of Pd5L10 was also detected starting from the addition of 2 eq. of NO3−. All of these intermediates start to drop in contents after the most stable Pd3L6 complex evolved in the system. We hypothesize that NO3− anions are acting as hydrogon-bonding anchors which pull together the neighbouring Pd2L2 square units on the framework, leading to a gradually shrinking of the ring size. The contents of Pd6L12 and Pd4L8 are aboundent during the transformation proecess, possibly due to the fact that they have even numbers of Pd2L2 square units, which can maximize the number of H bonding with the NO3− anchors. In contrast, the concentration of the Pd5L10 intermediate, which has odd number of Pd2L2 square units, was rather low (Supplementary Fig. 71).

In summary, a dynamic anion-adaptive self-assembly system was constructed consisting of a very simple ligand and PdII ions. The anion-induced transformation between the PdL2 species are reminiscent of the induced-fit guest-binding mechanism observed in nature. Moreover, this new class of donut-shaped assemblies provide unique tunable hydrogen-binding pockets, where we envisage molecular sensing/catalysis is possible to take place.

Methods

Materials

Unless otherwise noted, all chemicals and solvents were purchased from commercial corporations and used without further purification. Deuterated solvents were purchased from Admas and J&K scientific.

NMR measurements

1D and 2D NMR spectra were measured on a Bruker-BioSpin AVANCE Ш HD (400 MHz) spectrometer. 1H NMR chemical shifts were determined with respect to residual solvent signals of the deuterated solvents used. DOSY spectra were applied to estimate the dynamic radius for the compounds 2, 3, 4, 5 and 6 according to the Stokes-Einstein equation (1). Where: D is diffusion coefficient obtained from DOSY spectrum, K is Boltzmann constant, T is temperature, viscosity η was tested to be 2.2 mPa s and r is the estimated dynamic radius.

MS measurements

ESI-Q-TOF mass spectra were recorded on an Impact II UHR-TOF mass spectrometry from Bruker, with ESI-L low concentration tuning mix (from Agilent Technologies) as the internal standard (Accuracy <3 p.p.m.). Data analyses and simulations of ESI-TOF mass spectra were processed on a Bruker Data Analysis software (Version 4.3). Molecular mechanical structure simulations were performed on a Material Studio v6.0 software using the build-in geometry optimization task based on the Universal forcefield.

Synthesis and characterization

(PdL)(NO) (2) Ligand 1 (ref. 51) (4.98 mg, 20.07 μmol) was treated with Pd(NO3)2 (10.04 μmol) in [D6]DMSO (1 ml) at 70 °C for 5 h. 1H NMR confirmed the quantitative formation of complex 2. 1H NMR (400 MHz, [D6]DMSO, 298 K) δ 8.99 (s, 12H), 7.90 (d, J=8.3 Hz, 12H), 7.66 (d, J=8.3 Hz, 12H), 7.39 (t, J=7.8 Hz, 12H), 7.28 (t, J=7.8 Hz, 12H), 7.09 (d, J=13.7 Hz, 6H), 6.55 (d, J=14.0 Hz, 6H). 13C NMR (100 MHz, [D6]DMSO, 298 K) δ 145.39, 138.88, 130.67, 125.99, 124.90, 117.77, 111.82 and 54.51(Supplementary Figs 1–3). ESI-TOF-MS (NO3−salt, CH3CN): the following picked signals are those at the highest intensities. m/z Calcd for [Pd3L6(NO3−)4]2+ 1028.1513, found 1028.1468; Calcd for [Pd3L6(NO3−)3]3+ 665.1049, found 665.1049 (Supplementary Fig. 5).

(PdL)(BF) (5) PdCl2 (17.73 mg, 0.10 mmol) was dissolved in [D6]DMSO (2 ml) and stirred for 10 h at room temperature with AgBF4 (38.94 mg, 0.20 mmol). After removal of AgCl by filtration, the Pd(BF4)2 stock solution in [D6]DMSO was obtained quantitatively. Ligand 1 (20.00 mg, 80.60 μmol) was treated with Pd(BF4)2 (40.30 μmol) in [D6]DMSO (1 ml) at 70 °C for 5 h. After addition of 0.3 ml DCM into the solution, a small amount of precipitation appeared which was then filtrated off and pure compound 5 in [D6]DMSO was obtained after removal of DCM. The isolation yield of this compound is about 65%. 1H NMR (400 MHz, [D6]DMSO, 298 K) δ 9.06 (s, 12H), 9.00 (s, 12H), 8.17 (d, J=8.2 Hz, 12H), 7.63 (dd, J=17.0, 8.0 Hz, 36H), 7.35 (t, J=7.8 Hz, 12H), 7.22 (d, J=14.6 Hz, 12H), 6.99 (t, J=7.8 Hz, 12H), 6.84 (t, J=7.5 Hz, 12H), 6.57 (d, J=14.8 Hz, 12H), 6.09 (d, J=8.2 Hz, 12H). 19F NMR (376 MHz, [D6]DMSO, 298 K) δ -147.31. 13C NMR (100 MHz, [D6]DMSO, 298 K) δ 146.12, 144.82, 138.32, 138.20, 132.34, 128.63, 126.92, 126.53, 126.02, 125.64, 117.88, 117.39, 111.96, 110.55 and 54.36 (Supplementary Figs 6–11). ESI-TOF-MS (BF4−salt, CH3CN): the following picked signals are those at the highest intensities. m/z Calcd for [(Pd6L12)(BF4)9]3+ 1466.2455, found 1466.2397; Calcd for [(Pd6L12)(BF4)8]4+ 1077.9331, found 1077.9288; Calcd for [(Pd6L12)(BF4)7]5+ 844.9456, found 844.9425; Calcd for [(PdL12)(BF4)6]6+ 689.7873, found 689.7848 (Supplementary Fig. 12).

(PdL)(PF) (6a) PdCl2 (17.73 mg, 0.10 mmol) was dissolved in DMSO (2 ml) and stirred for 10 h at room temperature in dark with AgPF6 (50.57 mg, 0.20 mmol). After removal of AgCl by filtration, stock solution of Pd(PF6)2 in [D6]DMSO was obtained quantitatively. Then ligand 1 (20 mg, 80.60 μmol) was treated with Pd(PF6)2 (40.30 μmol) in [D6]DMSO (1 ml) at 70 °C for 5 h. 1H NMR confirmed the quantitative formation of complex. 1H NMR confirmed the quantitative formation of complex 6a. 1H NMR (400 MHz, [D6]DMSO, 298 K) δ 9.09 (s, 14H), 9.00 (s, 14H), 8.22 (d, J=8.4 Hz, 14H), 7.76 (d, J=8.2 Hz, 14H), 7.63 (t, J=7.5 Hz, 14H), 7.42 (d, J=12.7 Hz, 14H), 7.35 (d, J=8.4 Hz, 14H), 7.30–7.22 (m, 14H), 7.04 (t, J=7.6 Hz, 14H), 6.89 (t, J=7.6 Hz, 14H), 6.41 (d, J=13.7 Hz, 14H), 6.02 (d, J=8.2 Hz, 14H). 13C NMR (100 MHz, [D6]DMSO, 298 K) δ 145.32, 144.72, 138.06, 137.49, 132.14, 128.38, 126.81, 125.89, 117.40, 116.90, 111.79, 110.31 and 54.16 (Supplementary Figs 13–15). ESI-TOF-MS (PF6−salt, CH3CN): the following picked signals are those at the highest intensities. m/z Calcd for [(Pd7L14)(PF6)11]3+ 1938.4758, found 1938.4852; Calcd for [(Pd7L14)(PF6)10]4+ 1417.6157, found 1417.6235; Calcd for [(Pd7L14)(PF6)9]5+ 1105.0996, found 1105.1060; Calcd for [(Pd7L14)(PF6)8]6+ 896.7555, found 896.7605; Calcd for [(Pd7L14)(PF6)7]7+ 747.9384, found 747.9432; Calcd for [(Pd7L14)(PF6)6]8+ 636.3255, found 636.3299 (Supplementary Fig. 20).

(PdL)(CFSO) (6b) PdCl2 (17.73 mg, 0.10 mmol) was dissolved in [D6]DMSO (2 ml) and stirred for 10 h at room temperature in dark with AgCF3SO3 (51.39 mg, 0.20 mmol). After removal of AgCl by filtration, the stock solution of Pd(CF3SO3)2 in [D6]DMSO was obtained quantitatively. Then ligand 1 (10 mg, 40.29 μmol) was treated with Pd(CF3SO3)2 (20.15 μmol) in [D6]DMSO (1 ml) at 70 °C for 5 h. 1H NMR confirmed the quantitative formation of complex 6b. 1H NMR (400 MHz, [D6]DMSO, 298 K) δ 9.19 (d, J=8.6 Hz, 28H), 8.21 (d, J=8.2 Hz, 14H), 7.72 (d, J=7.9 Hz, 14H), 7.65–7.55 (m, 14H), 7.40–7.28 (m, 28H), 7.27–7.17 (m, 14H), 7.07–6.96 (m, 14H), 6.91–6.80 (m, 14H), 6.53 (d, J=14.5 Hz, 14H), 6.00 (d, J=7.8 Hz, 14H). 13C NMR (100 MHz, [D6]DMSO, 298 K) δ 145.32, 144.72, 138.06, 137.49, 132.14, 128.38, 126.81, 125.89, 117.40, 116.90, 111.79, 110.31 and 54.16 (Supplementary Figs 16–18). ESI-TOF-MS (CF3SO3− salt, CH3CN): the following picked signals are those at the highest intensities. m/z Calcd for [(Pd7L14)(CF3SO3)11]3+ 1953.4308, found 1953.4414; Calcd for [(Pd7L14)(CF3SO3)10]4+ 1427.8350, found 1427.8426; Calcd for [(Pd7L14)(CF3SO3)9]5+ 1112.4775, found 1112.4827; Calcd for [(Pd7L14)(CF3SO3)8]6+ 902.2392, found 902.2435; Calcd for [(Pd7L14)(CF3SO3)7]7+ 751.9261, found 751.9297; Calcd for [(Pd7L14)(CF3SO3)6]8+ 751.9261, found 751.9297; Calcd for [(Pd7L14)(CF3SO3)5]9+ 639.3163, found 639.3199 (Supplementary Fig. 21).

(PdL)(SO)(BF) (3) the solution of 5 (6.72 μmol) was treated with tetrabutylammonium hydrogen sulfate (10.27 mg, 30.24 μmol, 4.50 eq.) at 70 °C for 3 h. 1H NMR confirmed the quantitative formation of complex 3. 1H NMR (400 MHz, [D6]DMSO, 298 K) δ 10.46 (s, 16H), 9.30 (d, J=8.4 Hz, 16H), 8.06 (d, J=8.5 Hz, 16H), 7.46 (t, J=7.7 Hz, 16H), 7.36 (t, J=7.6 Hz, 16H), 7.16 (d, J=15.4 Hz, 8H), 7.07 (d, J=14.8 Hz, 8H). 13C NMR (100 MHz, DMSO, 298 K) δ 145.61, 138.53, 132.16, 125.77, 124.92, 118.68, 112.05 and 57.76 (Supplementary Figs 22–25). ESI-TOF-MS (SO42−/BF4−salt, CH3CN): the following picked signals are those at the highest intensities. m/z Calcd for [(Pd4L8)(SO4)2(BF4)2]2+ 1388.6898, found 1388.6908; Calcd for [(Pd4L8)(SO4)2(BF4)]3+ 896.7918, found 896.7930; Calcd for [(Pd4L8)(SO4)2]4+ 650.8428, found 650.8434 (Supplementary Figs 27 and 28).

(PdL)(HMoO)(BF) (4) the solution of 5 (6.72 μmol) was treated with tetrabutylammonium heptamolybdate (13.48 mg, 5.38 μmol, 0.80 eq.) at 70 °C for 3 h. 1H NMR confirmed the quantitative formation of complex 4. 1H NMR (400 MHz, [D6]DMSO, 298 K) δ 10.01 (ddd, J=65.3, 61.9, 39.5 Hz, 20H), 9.28 (t, J=28.2 Hz, 20H), 8.21–7.91 (m, 20H), 7.42 (d, J=8.0 Hz, 20H), 7.37 (s, 20H), 7.18 (s, 10H), 6.91 (s, 10H). 13C NMR (100 MHz, DMSO,298 K) δ 144.61, 138.26, 130.82, 125.10, 124.49, 119.10 and 111.60 (Supplementary Figs 29–33). ESI-TOF-MS (HMo7O245−, BF4−salt, CH3CN): the following picked signals are those at the highest intensities. m/z Calcd for [(Pd5L10)(HMo7O24)]5+ 814.3625, found 814.3622; Calcd for [(Pd5L10)(HMo7O24)(BF4)]4+ 1039.7043, found 1039.7051; Calcd for [(Pd5L10)(HMo7O24)(BF4)2]3+ 1414.9399, found 1414.9400 (Supplementary Figs 35 and 36).

Transformation of 6a into 5 by BF: 35 eq. of N(C4H9)4BF4 was added into the prepared solution of compound 6a, followed by stirring for 3 h at 70 °C. 1H NMR and ESI-TOF-MS spectra revealed that compound 6a changed into 5 (with mixed counter anions of PF6− and BF4−) in 80% yield (Supplementary Figs 42–44 and Supplementary Table 5).

Transformation of 6a into 4 by MoO: 0.8 eq. of [N(C4H9)4]6Mo7O24 was added into the prepared solution of compound 6a, followed by stirring for 3 h at 70 °C. 1H NMR and ESI-TOF-MS spectra revealed that compound 6a quantitatively changed into 4 (with mixed counter anions of PF6− and HMo7O245−) (Supplementary Figs 36 and 45).

Transformation of 6a into 3 by HSO: 5.25 eq. of N(C4H9)4HSO4 was added into the prepared solution of compound 6a, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed that the compound 6a quantitatively changed into 3 (with mixed counter anions of PF6− and SO4−) completely (Supplementary Figs 28 and 46).

Transformation of 6a into 2 by NO: 30 eq. of KNO3 was added into the prepared solution of compound 6a, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed the compound 6a quantitatively changed into 2 (with mixed counter anions of PF6− and NO3−) completely (Supplementary Figs 47 and 48).

Transformation of 5 into 4 by MoO: 0.8 eq. of [N(C4H9)4]6Mo7O24 was added into the prepared solution of compound 5, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed that compound 5 quantitatively changed into 4 (with mixed counter anions of BF4− and HMo7O245−) (Supplementary Figs 49–35).

Transformation of 5 into 3 by HSO: 4.5 eq. of N(C4H9)4HSO4 was added into the prepared solution of compound 5, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed the compound 5 quantitatively changed into 3 (with mixed counter anions of BF4− and SO4−) (Supplementary Figs 27 and 50).

Transformation of 5 into 2 by NO: 12 eq. of KNO3 was added into the prepared solution of compound 5, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed the compound 5 quantitatively changed into 2 (with mixed counter anions of BF4− and NO3−) (Supplementary Figs 51 and 52).

Transformation of 3 into 4 by MoO: 2.4 eq. of [N(C4H9)4]6Mo7O24 was added into the prepared solution of compound 3, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed the compound 3 quantitatively changed into 4 (with mixed counter anions of HSO4−, BF4− and HMo7O245−) (Supplementary Figs 53 and 54).

Transformation of 3 into 2 by NO: 13.3 eq. of KNO3 was added into the prepared solution of compound 3, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed the compound 3 quantitatively changed into 2 (with mixed counter anions of HSO4−, BF4− and NO3−) (Supplementary Figs 56 and 55).

Transformation of 2 into 4 by MoO: 2 eq. of [N(C4H9)4]6Mo7O24 was added into the prepared solution of compound 2, followed by stirring for 3 h at 70 °C. The 1H NMR and ESI-TOF-MS spectra revealed that compound 2 quantitatively changed into 4 (with mixed counter anions of NO3− and HMo7O245−) (Supplementary Figs 57 and 58).

Single crystal X-ray diffractions

The X-ray diffraction studies for complexes 2, 3, 4, 5 were carried out at the BL17B macromolecular crystallography beamline in SSRF. The collected diffraction data were processed with the HKL2000 software program52. All the structures were solved by direct methods and refined by full-matrix least-squares on F2 with anisotropic displacement using the SHELX software package53.

The crystals of these kinds of giant supramolecular assemblies often diffract very weekly in nature. Some of the final R factors were converged to very high values, because the crystal was diffracting very weakly due to a large amount of disordered/amorphous solvents and anions that could not be fully located. The electron residuals in such cases were removed by the SQUEEZE routine54. We tried our best to finish the refinement but still some A-alerts are found by the (IUCr) checkCIF routine, all of which are due to the poor diffraction nature of the crystals or the disorder of the solvents and anions. Details on crystal data collection and refinement were summarized in Supplementary Tables 1–4.

Crystal data for 2: space group P-1, a=18.088(4) Å, b=18.299(4) Å, c=20.871(4) Å, V=6,099(3) Å3, Z=2, T=80 K. Anisotropic least-squares refinement for the framework atoms and isotropic refinement for the other atoms on 30,186 independent merged reflections (Rint=0.0292) converged at residual wR2=0.3805 for all data; residual R1=0.1126 for 59494 observed data [I>2σ(I)], and goodness of fit (GOF)=1.048.

Crystal data for 3: space group P21c, a=18.178(4) Å, b=32.042(6) Å, c=18.582(4) Å, V=10,616(4) Å3, Z=2, T=293 K. Anisotropic least-squares refinement for the framework atoms and isotropic refinement for the other atoms on 26,904 independent merged reflections (Rint=0.0809) converged at residual wR2=0.2780 for all data; residual R1=0.0765 for 93,650 observed data [I>2σ(I)], and goodness of fit (GOF)=1.042.

Crystal data for 4: space group Pmmn, a=20.3994(4) Å, b=21.2991(4) Å, c=29.5319(6) Å, V=12,831.3(4) Å3, Z=2, T=293 K. Anisotropic least-squares refinement for the framework atoms and isotropic refinement for the other atoms on 13,683 independent merged reflections (Rint=0.0591) converged at residual wR2=0.5449 for all data; residual R1=0.1540 for 18,8179 observed data [I>2σ(I)], and goodness of fit (GOF)=2.991.

Crystal data for 5: space group R-3c, a=71.246(4) Å, b=71.246(4) Å, c=27.0831(16) Å, V=119056(15) Å3, Z=18, T=293 K. Anisotropic least-squares refinement for the framework atoms and isotropic refinement for the other atoms on 10,282 independent merged reflections (Rint=0.145) converged at residual wR2=0.4507 for all data; residual R1=0.1369 for 96,189 observed data [I>2σ(I)], and goodness of fit (GOF)=2.044.

Data availability

The X-ray crystallographic coordinates for structures reported in this article have been deposited at the Cambridge Crystallographic Data Centre (CCDC), under deposition nos CCDC 1529252-1529255. These data can be obtained free of charge (http://www.ccdc.cam.ac.uk/data_request/cif). All other data are either provided in the Article and its Supplementary Information or are available on request.

Additional information

How to cite this article: Zhang, T. et al. Adaptive self-assembly and induced-fit transformations of anion-binding metal-organic macrocycles. Nat. Commun. 8, 15898 doi: 10.1038/ncomms15898 (2017).

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