Lin Zhao1, Junli Zhao2, Jiajia Yu3, Rui Sun3, Xiaofeng Zhang1, Shuhua Hu1. 1. Orthopaedic Department, Jinshan Branch of the Sixth People's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 201500, China. 2. Department of Nephrology, Shanghai ZhouPu Hospital, Shanghai 201318, China. 3. Orthopaedic Institute, the Second Hospital of Lanzhou University, Lanzhou 730030, China.
Abstract
AIM: The aim of the study was to evaluate the efficacy of tissue-engineered periosteum (TEP) in repairing allogenic bone defects in the long term. MATERIALS & METHODS: TEP was biofabricated with osteoinduced rabbit bone marrow mesenchymal stem cells and porcine small intestinal submucosa (SIS). A total of 24 critical sized defects were created bilaterally in radii of 12 New Zealand White rabbits. TEP/SIS was implanted into the defect site. Bone defect repair was evaluated with radiographic and histological examination at 4, 8 and 12 weeks. RESULTS: Bone defects were structurally reconstructed in the TEP group with mature cortical bone and medullary canals, however this was not observed in the SIS group at 12 weeks. CONCLUSION: The TEP approach can effectively restore allogenic critical sized defects, and achieve maturity of long-bone structure in 12 weeks in rabbit models.
AIM: The aim of the study was to evaluate the efficacy of tissue-engineered periosteum (TEP) in repairing allogenic bone defects in the long term. MATERIALS & METHODS:TEP was biofabricated with osteoinduced rabbit bone marrow mesenchymal stem cells and porcine small intestinal submucosa (SIS). A total of 24 critical sized defects were created bilaterally in radii of 12 New Zealand White rabbits. TEP/SIS was implanted into the defect site. Bone defect repair was evaluated with radiographic and histological examination at 4, 8 and 12 weeks. RESULTS:Bone defects were structurally reconstructed in the TEP group with mature cortical bone and medullary canals, however this was not observed in the SIS group at 12 weeks. CONCLUSION: The TEP approach can effectively restore allogenic critical sized defects, and achieve maturity of long-bone structure in 12 weeks in rabbit models.
Entities:
Keywords:
allograft; bone defect; bone tissue engineering; in vivo bioreactor; rabbit
Authors: Chen Tao; Xie Lina; Wang Changxuan; Luo Cong; Yang Xiaolan; Huang Tao; An Hong Journal: J Biomed Mater Res B Appl Biomater Date: 2019-10-12 Impact factor: 3.368